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| - | {{:Team:St_Andrews/Template:Header}}
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| - | <html>
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| - | <head>
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| - | <style type="text/css">
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| - | body {
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| - | padding-top: 60px; /* 60px to make the container go all the way to the bottom of the topbar */
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| - | }
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| - | h1 {
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| - | padding-bottom: 10px;
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| - | }
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| - | #footer{
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| - | padding-top: 60px;
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| - | }
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| - | </style>
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| - |
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| - | <title></title>
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| - | </head>
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| - |
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| - | <body id="body-pattern">
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| - | <div class="container" id="content-container">
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| - | <header class="jumbotron subhead" id="overview">
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| - | <h1>Lab Book</p>
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| - | <p></p>
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| - | <h2>Procedure</h2>
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| - |
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| - | </header>
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| - |
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| - | <div class="tabbable">
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| - | <ul class="nav nav-tabs">
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| - | <li class="active"><a href="#tab1" data-toggle="tab">General Methods</a></li>
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| - | <li><a href="#tab2" data-toggle="tab">ω-3 Synthesis</a></li>
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| - | <li><a href="#tab3" data-toggle="tab">Metal-binding peptides</a></li>
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| - | </ul>
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| - |
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| - | <div class="tab-content">
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| - |
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| - | <div class="tab-pane active" id="tab1">
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| - |
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| - | <dl class="dl-horizontal">
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| - | <dt>date</dt>
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| - | <dd>what we did</dd>
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| - | <dt>date</dt>
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| - | <dd>what we did</dd>
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| - | <dt>date</dt>
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| - | <dd>what we did</dd>
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| - | </dl>
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| - |
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| - | <p>anyone really keen to write this up, up to protein visualization? Don't forget to mention the differing restriction enzymes and vectors of each team. I can add in links to the protocols page.</p>
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| - | <p>Please also refer to our <a href="https://2012.igem.org/Team:St_Andrews/Lab-book"><font color="gray">Protocols</font></a> page.</p>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - |
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| - | </div>
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| - |
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| - | <div class="tab-pane" id="tab2">
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| - | <BR> </BR>
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| - |
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| - | <span class="label"><a href="#Sequences"><font color="white">Sequences</font></a></span> <span class="label"><a href="#Primers"><font color="white">Primers</font></a></span> <span class="label"><a href="#Sequencing"><font color="white">Sequencing results</font></a></span> <span class="label"><a href="#Lipid"><font color="white">Lipid analysis</font></a></span>
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| - |
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| - | <BR> </BR>
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| - |
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| - | <p>Start from lipid extraction</p>
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| - | <p>primers, sequence results</p>
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| - |
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| - | <BR> </BR>
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| - |
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| - |
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| - | <a name="Sequences"><span class="label">Sequences</span></a>
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| - | <BR> </BR>
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| - | <p>
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| - | <div class="btn-toolbar">
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| - | <a data-toggle="modal" href="#modal1" class="btn btn-primary btn-small">Δ12 T. Cruzi</a>
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| - | <div class="modal hide" id="modal1">
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| - | <div class="modal-header">
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| - | <button type="button" class="close" data-dismiss="modal">×</button>
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| - | <h3>Δ12 T. Cruzi</h3>
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| - | </div>
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| - | <div class="modal-body">
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| - | <p>atgtcgcgggttgacaatttaactgtcgcgccgggtccaccggatgtcatgaaggcggtgcttaagtctgggcgcaacaaggagcacaat
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| - | gtcattgtgccttcctccgcactgaccattggagaaattcaggagaaaatcccggtaaagtactttgaacgcaacactacccggagcgtc
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| - | atgtttcttttacgcgacctcgcacaggtggcgttgacgtacgccattatgtacgccgtcgccttgccacttgccacttccatggaagtttctg
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| - | cagcaagaacgttggccgacggtggggtgttatcattgatggcaggtacagccatgacgacagcggcgtggcttttaaaggcagtgttgtg
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| - | ggcggtgttttggtttgtacagggacttaacggcactgcgctttgggtcctggctcacgaatgcggccatcaggcgttcagccccatgaaag
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| - | gtttgaatgacgccgttggcatgattttgcactctgcgctgctcgtgccgtaccacagctggcgcatcacccacggcggccaccacaaac
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| - | acacgaatcacctcacgaaggatcttgtgtttgttccagacaaacgaaacgcggttgtggagctcgtggaggaggcgccgctggtgttatt
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| - | aattcaattattgctgatttttctctttggttggccggcacatcttctttttaatgcctctggacaggaatttggccgactcgcgagccactttgac
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| - | cccggcgctccatttttccgcagcgaagaccgtcacgatattgtcctgtcgaatgttgggattgtcagcgcgttatttgtcattttctccagcgt
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| - | ttaccgctttggttttacaaatgttttctgctggtacattgtaccgtacctctgggtgaacttttggcttgtgtacattacatacctgcagcacacg
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| - | gatatacgcattcctcactacacacatgagcactggacgtttgttcgcggtgcattggcggctgtggacagggactacggctttgtccttaac
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| - | acatggctccatcacatcaatgattcccacgtggtacatcacctctttagcaaaatgccacactacaacgcaatccaggtgacaagaaag
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| - | tacattcgtgagatactgggtgccacatacattacggatgagaggtcactgtggaagatgctctgggaacagcgtagagagtgccgctatg
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| - | ttgttcccgcagagggcgtctgtgtctttcatgggtaa</p>
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| - | </div>
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| - | <div class="modal-footer">
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| - | <a href="#" class="btn" data-dismiss="modal">Close</a>
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| - | </div>
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| - | </div>
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| - |
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| - | <a data-toggle="modal" href="#modal2" class="btn btn-primary btn-small">Δ12 Synechocystis</a>
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| - | <div class="modal hide" id="modal2">
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| - | <div class="modal-header">
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| - | <button type="button" class="close" data-dismiss="modal">×</button>
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| - | <h3>Δ12 Synechocystis</h3>
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| - | </div>
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| - | <div class="modal-body">
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| - | <p>atgactgccacgattcccccgttgacaccaacggtaacgcccagcaatcccgatcgcccg
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| - | attgcggatctcaaactacaagacatcattaaaaccctgcccaaggaatgcttcgagaaa
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| - | aaagcgagcaaagcctgggcttctgttttgattaccctaggggcgatcgccgtgggctat
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| - | ttgggcattatttatctgccctggtactgcttgcccattacctggatctggacagggaca
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| - | gccttaacgggggccttcgttgtcggccatgactgtggccatcgctcctttgctaaaaaa
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| - | cgctgggtcaatgatttagtgggacatatcgcttttgctcccctcatctaccctttccat
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| - | agctggcgcctactccacgaccaccatcacctccacaccaacaaaattgaggttgataac
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| - | gcctgggatccctggagtgtggaagctttccaagccagcccggcgatcgtccggcttttt
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| - | tatcgggccatccggggtcccttctggtggactggttccattttccattggagcttaatg
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| - | cacttcaaactttccaactttgcccaaagggaccgcaataaagtcaaattatccattgcc
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| - | gttgtcttcctgtttgcggcgatcgcctttcctgccctaattatcaccacaggggtgtgg
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| - | ggtttcgtcaaattttggctaatgccctggttggtgtatcacttttggatgagcactttt
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| - | accattgtgcaccacaccattcccgaaattcgtttccgtcccgccgccgattggagtgcc
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| - | gccgaagcccagttaaatggtactgttcactgcgattatccccgttgggtggaagtgctc
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| - | tgccatgacatcaacgtccatattccccaccacctctccgttgccatcccttcctataac
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| - | ctacgactagcccacggaagtttaaaagaaaactggggaccttttctttacgagcgcacc
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| - | tttaactggcaattaatgcaacaaattagtgggcaatgtcatttatatgaccccgaacat
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| - | ggctaccgcaccttcggctccctgaaaaaagtttaa
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| - | </p>
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| - | </div>
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| - | <div class="modal-footer">
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| - | <a href="#" class="btn" data-dismiss="modal">Close</a>
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| - | </div>
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| - | </div>
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| - |
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| - | <a data-toggle="modal" href="#modal3" class="btn btn-primary btn-small">Δ15 Synechocystis</a>
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| - | <div class="modal hide" id="modal3">
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| - | <div class="modal-header">
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| - | <button type="button" class="close" data-dismiss="modal">×</button>
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| - | <h3>Δ15 Synechocystis</h3>
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| - | </div>
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| - | <div class="modal-body">
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| - | <p>gtgcgtctagaaatttcatcgcctcaaacaaagcttccttaccccaaaactgaagaatta
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| - | ccatttaccctccaagagctcagaaacgctattccagcggattgttttgagccatcggta
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| - | gtccggtccttgggctacttttttttggatgttggtttaattgccgggttttatgctcta
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| - | gcggcctaccttgattcctggttcttctatccgattttttggttaattcagggaacccta
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| - | ttctggtccctgtttgtggtgggccatgattgtggccatggctccttttccaaatccaaa
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| - | acccttaataattggattggtcatctcagccacacgccaattttggtgccttaccatggc
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| - | tggcgtattagtcatcgtactcaccatgccaacacgggcaatatcgacaccgacgaaagt
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| - | tggtatccagtgtcggagcaaaaatataaccaaatggcctggtatgaaaaacttctacgt
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| - | ttttacttgcctctgatcgcctaccccatttatctatttcggcgatcgccaaaccggcaa
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| - | ggctcccatttcatgcccggcagtcccctattccgtcccggagaaaaagcagctgttctc
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| - | accagcacctttgcccttgcagcctttgtcggcttccttggctttttaacttggcaattt
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| - | ggctggctatttttgctgaaattttatgttgccccctacctcgtgtttgtggtgtggtta
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| - | gatttggtcacatttttacatcacactgaagacaatatcccttggtatcgtggtgatgac
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| - | tggtattttctcaaaggtgccctctccaccattgatcgggattacggcttcattaacccc
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| - | attcaccatgacattggcacccacgtcgcccaccatattttctcgaatatgccccactac
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| - | aagttacgccgggcgactgaagccatcaagcccattttaggggaatattatcgatattct
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| - | gacgagccaatttggcaagctttttttaagtcctactgggcttgccattttgttcctaat
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| - | caaggttcaggggtctattaccaatccccatccaatggtggatatcaaaagaaaccttaa
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| - | </p>
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| - | </div>
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| - | <div class="modal-footer">
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| - | <a href="#" class="btn" data-dismiss="modal">Close</a>
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| - | </div>
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| - | </div>
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| - |
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| - | <a data-toggle="modal" href="#modal4" class="btn btn-primary btn-small">Δ6 Synechocystis</a>
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| - | <div class="modal hide" id="modal4">
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| - | <div class="modal-header">
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| - | <button type="button" class="close" data-dismiss="modal">×</button>
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| - | <h3>Δ6 Synechocystis</h3>
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| - | </div>
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| - | <div class="modal-body">
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| - | <p>atgctaacagcggaaagaattaaatttacccagaaacgggggtttcgtcgggtactaaac
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| - | caacgggtggatgcctactttgccgagcatggcctgacccaaagggataatccctccatg
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| - | tatctgaaaaccctgattattgtgctctggttgttttccgcttgggcctttgtgcttttt
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| - | gctccagttatttttccggtgcgcctactgggttgtatggttttggcgatcgccttggcg
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| - | gccttttccttcaatgtcggccacgatgccaaccacaatgcctattcctccaatccccac
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| - | atcaaccgggttctgggcatgacctacgattttgtcgggttatctagttttctttggcgc
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| - | tatcgccacaactatttgcaccacacctacaccaatattcttggccatgacgtggaaatc
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| - | catggagatggcgcagtacgtatgagtcctgaacaagaacatgttggtatttatcgtttc
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| - | cagcaattttatatttggggtttatatcttttcattcccttttattggtttctctacgat
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| - | gtctacctagtgcttaataaaggcaaatatcacgaccataaaattcctcctttccagccc
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| - | ctagaattagctagtttgctagggattaagctattatggctcggctacgttttcggctta
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| - | cctctggctctgggcttttccattcctgaagtattaattggtgcttcggtaacctatatg
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| - | acctatggcatcgtggtttgcaccatctttatgctggcccatgtgttggaatcaactgaa
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| - | tttctcacccccgatggtgaatccggtgccattgatgacgagtgggctatttgccaaatt
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| - | cgtaccacggccaattttgccaccaataatcccttttggaactggttttgtggcggttta
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| - | aatcaccaagttacccaccatcttttccccaatatttgtcatattcactatccccaattg
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| - | gaaaatattattaaggatgtttgccaagagtttggtgtggaatataaagtttatcccacc
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| - | ttcaaagcggcgatcgcctctaactatcgctggctagaggccatgggcaaagcatcgtga
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| - | </p>
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| - | </div>
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| - | <div class="modal-footer">
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| - | <a href="#" class="btn" data-dismiss="modal">Close</a>
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| - | </div>
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| - | </div>
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| - |
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| - | <a data-toggle="modal" href="#modal5" class="btn btn-primary btn-small">ELO 6 L. major</a>
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| - | <div class="modal hide" id="modal5">
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| - | <div class="modal-header">
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| - | <button type="button" class="close" data-dismiss="modal">×</button>
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| - | <h3>ELO 6 L. major</h3>
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| - | </div>
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| - | <div class="modal-body">
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| - | <p>atgaaggtcatcgtcgcttctggcccggacggtgcccgcaagcacgaggtggagctggca
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| - | gccaacgccacgctcgcagatctgaagaaggcctaccaacggggtgtggacgtgcaccgc
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| - | aagtcgttcaaggttcccagcgcggagtcgccgctgccaggtgcggatagtggcaagctg
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| - | cgcccgaacctcattactctgtcagataaggtgcccctgtcgcagcagggggtgaaggat
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| - | ggctcggtgatcacttacaaggacctcggcccgcagatcggctaccgcacggtgttctac
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| - | gtcgagtatgccggccccatcgccttcatgctgctgtacgccatgcgcccttcgctcatc
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| - | tacggctctgccccgatgccggcttacggctacacgcagaagctatacattggcctcttc
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| - | ctcgcccacttcttaaagcgcgagctcgagtccatgtttgtgcacaagttctcgcaccca
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| - | acgatgccgatgcgcaacatcttcaagaactgcatctactactggtccttcgccgccttc
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| - | atcggctacgtgctgtgcagtccttcattcacgccgaccagcaccatgcagtcaaacttc
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| - | ggcgccgtggtcatggtcatcaacgagctgctgaacttcgcggtgcactaccagcttagc
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| - | acgatgcgcaagtccgatggtgacaccacccgcaacgtgccgcaaggccctctgttcgcc
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| - | ttcgtctcgtgcccgaactacttctttgagattatgtcgtgggtgtccttttccatcggc
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| - | acaaatatgttatcctcctggttcttcacactcgccggtttcgtgcagatggcggactgg
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| - | gcgaagaagaagcaccggggctacgtcaaggcggacccggccaataaaaagaaggccgcc
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| - | attctgcccttcatcatgtag</p>
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| - | </div>
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| - | <div class="modal-footer">
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| - | <a href="#" class="btn" data-dismiss="modal">Close</a>
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| - | </div>
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| - | </div>
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| - |
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| - | <BR> </BR><BR> </BR>
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| - |
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| - | <a name="Primers"><span class="label">Primers</span></a>
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| - |
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| - | <BR> </BR>
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| - | <p>All primers are notated 5' to 3'.
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| - | <BR> </BR>
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| - | <div class="btn-toolbar">
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ12 T. Cruzi forward
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span3">ATATCATATGTGGCGGGTTGACAATTTA
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| - | </ul>
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| - | </div>
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| - |
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ12 T. Cruzi reverse
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <p><div class="span3">ATATCTCGAGTTACCCATGAAAGACAC</div></p>
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| - | </ul>
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| - | </div>
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| - |
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ12 Synechocystis forward
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span3">ATATCATATGACTGCCACGATTCCC</div>
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| - | </ul>
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| - | </div>
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| - |
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ12 Synechocystis reverse
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span3">ATATCTCGAGTTAAACTTTTTTCAGGGAGC</div>
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| - | </ul>
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| - | </div>
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| - |
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ15 Synechocystis forward
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span3">ATATCATATCGCTCTAGAAATTTCATC</div>
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| - | </ul>
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| - | </div>
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| - | </div>
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| - |
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| - | <p>
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| - | <div class="btn-toolbar">
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ15 Synechocystis reverse
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span3">ATATCACGACTTAAGGTTTCTTTTGATATC</div>
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| - | </ul>
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| - | </div>
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| - |
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ6 Synechocystis forward
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span3">ATATCATATGCAAACAGCGGAAAGAATT</div>
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| - | </ul>
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| - | </div>
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| - |
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ6 Synechocystis reverse
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span4">ATATCTCGAGTAACGATGCTTTGACCATGGCCTCTAG</div>
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| - | </ul>
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| - | </div></p>
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| - | </div>
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| - |
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| - | <BR> <?BR>
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| - | <p>When using the pET-duet vector, we needed additionally primers for the alternative restriction sites and His-tags.</p>
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| - |
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| - | <div class="btn-toolbar">
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
| |
| - | ELO6 L. major forward
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span6">TATCATATGGGAAGCAGCCATCATCATCATCATCACAGCATGGTCTCTCTGGAGCAGGCC</div>
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| - | </ul>
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| - | </div>
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| - |
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ15 T. Cruzi forward
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span6">TATCATATGGGCAGCCATCATCATCATCATCACAGCATGCGTCTAGAAATTTCATCG</div>
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| - | </ul>
| |
| - | </div>
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| - |
| |
| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ6 T. Cruzi forward
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span4">GGATCCGAATTCATCTAACAGCGGAAAGAATT</div>
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| - | </ul>
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| - | </div>
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| - |
| |
| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ6 T. Cruzi reverse
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span4">GACAAGCTTTCACGATGCTTTGCCCATGGCCTCTAC</div>
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| - | </ul>
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| - | </div>
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| - |
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ12 T. Cruzi forward
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| - | <span class="caret"></span>
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| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span4"> GGATCCGAATTCATGACTGCCACGATTCCC</div>
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| - | </ul>
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| - | </div>
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| - |
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| - | <div class="btn-group">
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| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
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| - | Δ12 T. Cruzi reverse
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| - | <span class="caret"></span>
| |
| - | </a>
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| - | <ul class="dropdown-menu">
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| - | <div class="span3">GACAAGCTTTTAAACTTTTTTCAGGGAGC</div>
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| - | </ul>
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| - | </div>
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| - | </div>
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| - | <BR> </BR>
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| - |
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| - |
| |
| - | <a name="Sequencing"><span class="label">Sequencing results</span></a>
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| - | <BR> </BR>
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| - |
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| - |
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| - |
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| - |
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| - |
| |
| - | <dl class="dl-horizontal">
| |
| - | <dt>date and gene</dt>
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| - | <dd>result</dd>
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| - | <dt>date and gene</dt>
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| - | <dd>result</dd>
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| - | <dt>date and gene</dt>
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| - | <dd>result</dd>
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| - | <dt>23/7/12 Δ12 Syn. in pET-15b and 20b</dt>
| |
| - | <dd><p><ul>15b forward gave 0 nucleotides</ul></p>
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| - | <p><ul>15b reverse gave 0 nucleotides</ul></p>
| |
| - | <p><ul>20b forward gave a 41 nucleotide result.</ul></p>
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| - |
| |
| - | <ul><div class="btn-group">
| |
| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">Sequence<span class="caret"></span>
| |
| - | </a>
| |
| - | <ul class="dropdown-menu">
| |
| - | <div class="span5"><font size="1">gCGnCctGntGCCgCGCGGCnnnnnnnnnnnnnnnnnngac</font></div>
| |
| - | </ul>
| |
| - | </div></ul>
| |
| - |
| |
| - | <p><ul>A BLAST search showed this to be compatible with <i>Crocosphaera watsonii</i> (genome shotgun sequence, 21%), a diazotrophic cyanobacteria. Add in relations to synechocystis.</ul></p>
| |
| - |
| |
| - | <p><ul>20b reverse gave 0 nucleotides</ul></p>
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| - |
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| - |
| |
| - |
| |
| - | </dd>
| |
| - | </dl>
| |
| - | </div>
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| - |
| |
| - | <BR> </BR>
| |
| - | <BR> </BR>
| |
| - |
| |
| - | <a name="Lipid"><span class="label">Lipid analysis</span></a>
| |
| - |
| |
| - | <BR> </BR><BR> </BR><BR> </BR><BR> </BR>
| |
| - | <BR> </BR><BR> </BR><BR> </BR><BR> </BR><BR> </BR>
| |
| - | </div>
| |
| - |
| |
| - | <div class="tab-pane" id="tab3">
| |
| - | <p>who knows what those Metal Mickies are up to. Eating nachos in the Whey Pat most likely. MEGA LOLS (all caps, with a Z).</p>
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| - |
| |
| - | <BR> </BR>
| |
| - |
| |
| - | <p><span class="label"><a href="#Primers2"><font color="white">Primers</font></a></span><span class="label"><a href="#Digestion2"><font color="white">Digestion</font></a></span><span class="label"><a href="#Transformation2"><font color="white">Transformation</font></a></span></p>
| |
| - |
| |
| - | <BR> </BR>
| |
| - | <a name="Primers2"><span class="label">Primers</span></a>
| |
| - |
| |
| - | <BR> </BR>
| |
| - | <p>All primers are notated 5' to 3'.
| |
| - | <BR> </BR>
| |
| - | <div class="btn-toolbar">
| |
| - | <div class="btn-group">
| |
| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
| |
| - | Ni forward
| |
| - | <span class="caret"></span>
| |
| - | </a>
| |
| - | <ul class="dropdown-menu">
| |
| - | <div class="span3">AATTCCATCATCACCATCACCACC</ul>
| |
| - | </div>
| |
| - |
| |
| - | <div class="btn-group">
| |
| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
| |
| - | Ni reverse
| |
| - | <span class="caret"></span>
| |
| - | </a>
| |
| - | <ul class="dropdown-menu">
| |
| - | <p><div class="span3">TCGAGGTGGTGATCGTGATGATGG</div></p>
| |
| - | </ul>
| |
| - | </div>
| |
| - |
| |
| - | <div class="btn-group">
| |
| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
| |
| - | Ni<sub>2</sub> forward
| |
| - | <span class="caret"></span>
| |
| - | </a>
| |
| - | <ul class="dropdown-menu">
| |
| - | <div class="span4">AATTCATGTGTACAACATGCGGTTGCGGTGAAGGCC</div>
| |
| - | </ul>
| |
| - | </div>
| |
| - |
| |
| - | <div class="btn-group">
| |
| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
| |
| - | Ni<sub>2</sub> reverse
| |
| - | <span class="caret"></span>
| |
| - | </a>
| |
| - | <ul class="dropdown-menu">
| |
| - | <div class="span4">TCGAGGCCTTCACCGCAACCGCATGTTGTACACATG</div>
| |
| - | </ul>
| |
| - | </div>.
| |
| - | </div>
| |
| - |
| |
| - | <p>
| |
| - | <div class="btn-toolbar">
| |
| - | <div class="btn-group">
| |
| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
| |
| - | Pd forward
| |
| - | <span class="caret"></span>
| |
| - | </a>
| |
| - | <ul class="dropdown-menu">
| |
| - | <div class="span3">AGCGTGACCCAGAACAAATAT</div>
| |
| - | </ul>
| |
| - | </div>
| |
| - |
| |
| - | <div class="btn-group">
| |
| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
| |
| - | Pd reverse
| |
| - | <span class="caret"></span>
| |
| - | </a>
| |
| - | <ul class="dropdown-menu">
| |
| - | <div class="span3">ATATTTGTTCTGGGTCACGCT</div>
| |
| - | </ul>
| |
| - | </div>
| |
| - |
| |
| - | <div class="btn-group">
| |
| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
| |
| - | Pt forward
| |
| - | <span class="caret"></span>
| |
| - | </a>
| |
| - | <ul class="dropdown-menu">
| |
| - | <div class="span3">GATCGCACCAGCACCTGGCGC</div>
| |
| - | </ul>
| |
| - | </div>
| |
| - |
| |
| - | <div class="btn-group">
| |
| - | <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
| |
| - | Pt reverse
| |
| - | <span class="caret"></span>
| |
| - | </a>
| |
| - | <ul class="dropdown-menu">
| |
| - | <div class="span3">GCGCCAGGTGCTGGTGCGATC</div>
| |
| - | </ul>
| |
| - | </div></p>
| |
| - | </div>
| |
| - |
| |
| - | <BR> </BR>
| |
| - |
| |
| - | <p>For primer annealing in the PCR, the primer sequences were combined in the following way:</p>
| |
| - |
| |
| - | <li><strong>GST Forward</strong> and <strong>Ni Reverse</strong></li>
| |
| - | <li><strong>GST Forward</strong> and <strong>Ni<sub>2</sub> Reverse</strong></li>
| |
| - | <li><strong>GST Forward</strong> and <strong>Pd Reverse</strong></li>
| |
| - | <li><strong>GST Forward</strong> and <strong>Pt Reverse</strong></li>
| |
| - |
| |
| - | <BR> </BR>
| |
| - | <a name="Digestion2"><span class="label">Digestion</span></a>
| |
| - |
| |
| - | <BR> </BR>
| |
| - | <p>pGEX-6P-1 was cut using EcoR1(954) and Xho1 (975)</p>
| |
| - |
| |
| - | <BR> </BR>
| |
| - |
| |
| - | <a name="Transformation2"><span class="label">Transformation</span></a>
| |
| - | <BR> </BR>
| |
| - | <p>pGEX vector with insert transformed into DH5α E. coli cells to replicate.</p>
| |
| - | <p>pGEX vector with G-Block insert transformed into DH5α E. coli cells to replicate.</p>
| |
| - | <BR> </BR>
| |
| - | <p>pGEX vector with insert transformed into BL21 E. coli cells for protein expression.</p>
| |
| - | <p>pGEX vector with G-Block insert transformed into BL21 E. coli cells for protein expression.</p>
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| - |
| |
| - |
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| - |
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
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| - | <BR> </BR>
| |
| - | <BR> </BR>
| |
| - | </div>
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| - | </div>
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| - |
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| - |
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| - |
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| - | </div>
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| - |
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| - | </body>
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| - | </html>
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| - | {{:Team:St_Andrews/Template:Footer}}
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