Team:Tec-Monterrey/antifreeze/data

From 2012.igem.org

(Difference between revisions)
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}
}
#image {
#image {
-
margin-top:10px;
+
width:640px;
-
width:570px;
+
height:480px;
-
min-width:570px;
+
margin:none;
-
max-width:570px;
+
padding:none;
-
height:385px;
+
-
min-height:385px;
+
-
max-height:385px;
+
}
}
#textinfo {
#textinfo {
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#div_subcontent #bt_design{ background:url(https://static.igem.org/mediawiki/igem.org/f/fa/TECMTY_partedex_design.png) no-repeat; border:none; height:60px; width:214px;}
#div_subcontent #bt_design{ background:url(https://static.igem.org/mediawiki/igem.org/f/fa/TECMTY_partedex_design.png) no-repeat; border:none; height:60px; width:214px;}
#div_subcontent #bt_design:hover{ background:url(https://static.igem.org/mediawiki/igem.org/e/eb/TECMTY_partedex_design_hover.png) no-repeat;}
#div_subcontent #bt_design:hover{ background:url(https://static.igem.org/mediawiki/igem.org/e/eb/TECMTY_partedex_design_hover.png) no-repeat;}
 +
#div_subcontent #bt_safety{ background:url(https://static.igem.org/mediawiki/igem.org/9/9d/TECMTY_partedex_safety.png) no-repeat; border:none; height:60px; width:214px;}
 +
#div_subcontent #bt_safety:hover{ background:url(https://static.igem.org/mediawiki/igem.org/d/df/TECMTY_partedex_safety_hover.png) no-repeat;}
.content #div_subcontent {
.content #div_subcontent {
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$('#tab2').click(function() {
$('#tab2').click(function() {
$('#replace').html($('#content2').html());
$('#replace').html($('#content2').html());
 +
prepareButtons2();
});
});
-
//Pokedex
+
//Pokedex Parts
-
var currentPart;
+
var currentPart = 0;
-
var textPart;
+
var textPart = 0;
var part_images = new Array();
var part_images = new Array();
var part_origin = new Array();
var part_origin = new Array();
var part_use = new Array();
var part_use = new Array();
var part_design = new Array();
var part_design = new Array();
 +
var part_safety = new Array();
 +
var struct_images = new Array();
 +
var struct_use = new Array();
 +
var struct_design = new Array();
 +
var struct_safety = new Array();
function prepareButtons() {
function prepareButtons() {
-
fillArrays();
 
//parts
//parts
$("div#div_subcontent #part1").click(function() {
$("div#div_subcontent #part1").click(function() {
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$("div#div_subcontent #bt_design").click(function() {
$("div#div_subcontent #bt_design").click(function() {
textPart = 2;
textPart = 2;
 +
changeStuff();
 +
});
 +
$("div#div_subcontent #bt_safety").click(function() {
 +
textPart = 3;
changeStuff();
changeStuff();
 +
});
 +
}
 +
function prepareButtons2() {
 +
alert("2");
 +
//constructs
 +
$("div#div_subcontent #part1").click(function() {
 +
currentPart = 0;
 +
textPart = 0;
 +
changeStuff2();
 +
});
 +
$("div#div_subcontent #part2").click(function() {
 +
currentPart = 1;
 +
textPart = 0;
 +
changeStuff2();
 +
});
 +
//buttons
 +
$("div#div_subcontent #bt_use").click(function() {
 +
textPart = 0;
 +
changeStuff2();
 +
});
 +
$("div#div_subcontent #bt_design").click(function() {
 +
textPart = 1;
 +
changeStuff2();
 +
});
 +
$("div#div_subcontent #bt_safety").click(function() {
 +
textPart = 2;
 +
changeStuff2();
});
});
}
}
function fillArrays() {
function fillArrays() {
-
currentPart = 0;
 
if (part_images.length == 0)
if (part_images.length == 0)
{
{
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part_images[3] = "https://static.igem.org/mediawiki/igem.org/8/85/TECMTY_parte11.gif";
part_images[3] = "https://static.igem.org/mediawiki/igem.org/8/85/TECMTY_parte11.gif";
-
part_origin[0] = "aflhkkfas";
+
part_origin[0] = "The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. This protein is produced natively in the beetle Raghium inquisitor. (Kristiansen E, 2011)";
-
part_origin[1] = "bflhkkfas";
+
part_origin[1] = "The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. The proU promoter is from E.coli and works activating transcription when the bacterium is osmotically stressed. (Kristiansen E, 2011)";
-
part_origin[2] = "cflhkkfas";
+
part_origin[2] = "The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. This protein is produced natively in the beetle Raghium inquisitor. This part includes the OmpA signal sequence native from E.coli and the pBAD L-arabinose inducible promoter. (Kristiansen E, 2011)";
-
part_origin[3] = "dflhkkfas";
+
part_origin[3] = "Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. This protein is produced natively in the beetle Raghium inquisitor. (Kristiansen E, 2011)";
 +
 
 +
part_use[0] = " ";
 +
part_use[1] = "This protein has an approximate weight of 13.7kDa and is better visualized in a SDS-PAGE with Tris-Tricine gel buffer, because of its low molecular weight. It can be purified by affinity with its 6xHis tag. An induction with 300mM of NaCl presents good expression. (Gasteiger E., 2005)";
 +
part_use[2] = "This protein has an approximate weight of 13.7kDa and is better visualized in a SDS-PAGE with Tris-Tricine gel buffer, because of its low molecular weight. It can be purified by affinity with its 6xHis tag. (Gasteiger E., 2005)";
 +
part_use[3] = " ";
-
part_use[0] = "1flhkkfas";
+
part_design[0] = "This part was designed by adding a 6xHis tag for affinity purification to the anti freeze protein from Raghium inquisitor. This part was codon optimized for its expression in E.coli.";
-
part_use[1] = "2flhkkfas";
+
part_design[1] = "The RiAFP is expressed when around 300mM NaCl added. This part was designed by adding a 6xHis tag for affinity purification to the anti freeze protein from Raghium inquisitor. This part was codon optimized for its expression in E.coli.";
-
part_use[2] = "3flhkkfas";
+
part_design[2] = "The RiAFP is expressed when around 0.1% L-Arabinose is added. This part was designed by adding a 6xHis tag for affinity purification to the anti freeze protein from Raghium inquisitor and the signal sequence of the ompA gene for periplasmic secretion. This part was codon optimized for its expression in E.coli.";
-
part_use[3] = "4flhkkfas";
+
part_design[3] = "This part was codon optimized for its expression in E.coli.";
-
part_design[0] = "dsadflhkkfas";
+
part_safety[0] = "The RiAFP has no reported toxicity or hazardousness, but its important to remember to have the basic biosafety precautions using a biosecurity chamber when handling any transformed strains.";
-
part_design[1] = "dasdsflhkkfas";
+
part_safety[1] = "The RiAFP has no reported toxicity or hazardousness, but its important to remember to have the basic biosafety precautions using a biosecurity chamber when handling any transformed strains. The inductor also presents no hazard as it is just common salt.";
-
part_design[2] = "fqweqwlhkkfas";
+
part_safety[2] = "The RiAFP has no reported toxicity or hazardousness, but its important to remember to have the basic biosafety precautions using a biosecurity chamber when handling any transformed strains. The inductor presents no hazard as is arabinose.";
-
part_design[3] = "flhkaskfas";
+
part_safety[3] = "The RiAFP has no reported toxicity or hazardousness, but it’s important to remember to have the basic biosafety precautions using a biosecurity chamber when handling any transformed strains.";
 +
}
 +
if (struct_images.length == 0)
 +
{
 +
struct_images[0] = "https://static.igem.org/mediawiki/2012/c/cb/TECMTY_Construct_afp_ri.png";
 +
struct_images[1] = "https://static.igem.org/mediawiki/2012/5/55/TECMTY_Construct_afp_ompari.png";
 +
 
 +
struct_use[0] = "This construct codes for Raghium inquisitor anti-freeze protein, and as such we are using it to protect the cell from being lysed by inhibiting the ice crystal growth. ";
 +
struct_use[1] = "This construct codes for Raghium inquisitor anti-freeze protein, and as such we are using it to protect the cell from being lysed by inhibiting the ice crystal growth. Additionaly, this construct makes the RiAFP go to the periplasm so its cryoprotection to the cell may change as well.";
 +
 
 +
struct_design[0] = "This construct acts as a generator for Raghium inquisitor anti freeze protein by osmotic pressure induction (NaCl induction). It also includes a 6xHis tag for purification. This expresses the RiAFP in the cytoplasm, acting as an intracellular cryopreservant.";
 +
struct_design[1] = "This construct acts as a generator for Raghium inquisitor anti freeze protein by arabinose induction. It also includes a 6xHis tag for purification. This exports the RiAFP to the periplasm, acting as cryopreservant from the periplasm.";
 +
 
 +
struct_safety[0] = "The RiAFP has no reported toxicity or hazardousness, but its important to remember to have the basic biosafety precautions using a biosecurity chamber when handling any transformed strains. The inductor also presents no hazard as it is just common salt.";
 +
struct_safety[1] = "The RiAFP has no reported toxicity or hazardousness, but its important to remember to have the basic biosafety precautions using a biosecurity chamber when handling any transformed strains. The inductor presents no hazard as is arabinose.";
}
}
}
}
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else if (textPart == 1)
else if (textPart == 1)
$('div#div_subcontent #textinfo').html(part_use[currentPart]);
$('div#div_subcontent #textinfo').html(part_use[currentPart]);
-
else
+
else if (textPart == 2)
$('div#div_subcontent #textinfo').html(part_design[currentPart]);
$('div#div_subcontent #textinfo').html(part_design[currentPart]);
 +
else
 +
$('div#div_subcontent #textinfo').html(part_safety[currentPart]);
}
}
 +
function changeStuff2()
 +
{
 +
$('div#div_subcontent #repImg').attr("src",struct_images[currentPart]);
 +
if (textPart == 0)
 +
$('div#div_subcontent #textinfo').html(struct_use[currentPart]);
 +
else if (textPart == 1)
 +
$('div#div_subcontent #textinfo').html(struct_design[currentPart]);
 +
else
 +
$('div#div_subcontent #textinfo').html(struct_safety[currentPart]);
 +
}
 +
//on load funcs
 +
$('#tab1').click();
 +
fillArrays();
});
});
</script>
</script>
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<img src="https://static.igem.org/mediawiki/igem.org/3/38/TECMTY_partedex_origin_hover.png" />
<img src="https://static.igem.org/mediawiki/igem.org/3/38/TECMTY_partedex_origin_hover.png" />
<img src="https://static.igem.org/mediawiki/igem.org/4/48/TECMTY_partedex_use_hover.png" />
<img src="https://static.igem.org/mediawiki/igem.org/4/48/TECMTY_partedex_use_hover.png" />
 +
<img src="https://static.igem.org/mediawiki/igem.org/0/00/TECMTY_parte14.gif" />
 +
<img src="https://static.igem.org/mediawiki/igem.org/1/1a/TECMTY_parte12.gif" />
 +
<img src="https://static.igem.org/mediawiki/igem.org/a/a4/TECMTY_parte13.gif" />
 +
<img src="https://static.igem.org/mediawiki/igem.org/8/85/TECMTY_parte11.gif" />
</span>
</span>
-
 
<div class="preload" id="content1">
<div class="preload" id="content1">
<h1>PARTS</h1>
<h1>PARTS</h1>
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<td width="60%">
<td width="60%">
<div id="image" class="inner">
<div id="image" class="inner">
-
<img src="" id="repImg"/>
+
<img src="https://static.igem.org/mediawiki/igem.org/0/00/TECMTY_parte14.gif" id="repImg"/>
</div>
</div>
</td>
</td>
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<tr>
<tr>
<td align="center"><button type="button" id="bt_design">&nbsp;</button></td>
<td align="center"><button type="button" id="bt_design">&nbsp;</button></td>
 +
</tr>
 +
<tr>
 +
<td align="center"><button type="button" id="bt_safety">&nbsp;</button></td>
</tr>
</tr>
</table>
</table>
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</table>
</table>
<div id="textinfo">
<div id="textinfo">
-
Herp a derp
+
The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. This protein is produced natively in the beetle Raghium inquisitor. (Kristiansen E, 2011)
</div>
</div>
</div>
</div>
<span class="preload" id="content2">
<span class="preload" id="content2">
 +
<h1>CONSTRUCTS</h1>
 +
<table width="100%">
 +
<tr>
 +
<td>&nbsp;</td>
 +
<td width="9%"><button type="button" id="part1">&nbsp;</button></td>
 +
<td width="9%"><button type="button" id="part2">&nbsp;</button></td>
 +
<td>&nbsp;</td>
 +
</tr>
 +
</table>
 +
<table>
 +
<tr>
 +
<td width="60%">
 +
<div id="image" class="inner">
 +
<img src="sagduakfe" id="repImg"/>
 +
</div>
 +
</td>
 +
<td width="40%">
 +
<div id="buttons">
 +
<table width="100%" align="center">
 +
<tr>
 +
<td align="center"><button type="button" id="bt_use">&nbsp;</button></td>
 +
</tr>
 +
<tr>
 +
<td align="center"><button type="button" id="bt_design">&nbsp;</button></td>
 +
</tr>
 +
<tr>
 +
<td align="center"><button type="button" id="bt_safety">&nbsp;</button></td>
 +
</tr>
 +
</table>
 +
</div>
 +
</td>
 +
</tr>
 +
</table>
 +
<div id="textinfo">
 +
This construct codes for Raghium inquisitor anti-freeze protein, and as such we are using it to protect the cell from being lysed by inhibiting the ice crystal growth.
 +
</div>
</span>
</span>
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</td>
</td>
<td width="25%">
<td width="25%">
-
<button type="button" id="tab2" class="tabcolor">&nbsp;</button>
+
<button type="button" id="tab2" class="tabcolor">constructs</button>
</td>
</td>
<td width="25%">
<td width="25%">

Revision as of 03:36, 23 September 2012

Tec Igem 2012 1 2 3 4 5 6 7 8 9 10 11 12

PARTS

   
The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. This protein is produced natively in the beetle Raghium inquisitor. (Kristiansen E, 2011)

CONSTRUCTS

   
This construct codes for Raghium inquisitor anti-freeze protein, and as such we are using it to protect the cell from being lysed by inhibiting the ice crystal growth.
project
Oh mah god

Retrieved from "http://2012.igem.org/Team:Tec-Monterrey/antifreeze/data"