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- | <html>
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| | | |
- | <script TYPE="text/javascript">
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- | <!--
| |
- | // copyright 1999 Idocs, Inc. http://www.idocs.com
| |
- | // Distribute this script freely but keep this notice in place
| |
- | function numbersonly(myfield, e, dec)
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- | {
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- | var key;
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- | var keychar;
| |
- |
| |
- | if (window.event)
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- | key = window.event.keyCode;
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- | else if (e)
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- | key = e.which;
| |
- | else
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- | return true;
| |
- | keychar = String.fromCharCode(key);
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- |
| |
- | // control keys
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- | if ((key==null) || (key==0) || (key==8) ||
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- | (key==9) || (key==13) || (key==27) )
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- | return true;
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- |
| |
- | // numbers
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- | else if ((("0123456789").indexOf(keychar) > -1))
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- | return true;
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- |
| |
- | // decimal point jump
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- | else if (dec && (keychar == "."))
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- | {
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- | myfield.form.elements[dec].focus();
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- | return false;
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- | }
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- | else
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- | return false;
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- | }
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- |
| |
- | //-->
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- | </script>
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- |
| |
- | <body>
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- | <form name="form1" action="http://igem2012.fri.uni-lj.si:443/cellulator/bistable" target="_blank" method="GET">
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- | <input type="hidden" name="check_submit" value="1"/>
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- | <hr />
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- | <b>INPUT VECTORS DOSAGE</b> <br/>
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- |
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- | <table>
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- | <th>
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- | <tr><td>Construct</td><td>Dosage</td></tr>
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- | </th>
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- | <tr><td><strong>[A]_pCMV_TAL-B:KRAB_BFP</strong> </td><td> <input type="text" name="plasmid1" size="5" value="190" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
| |
- | <tr><td><strong>[B]_pCMV_TAL-A:KRAB_mCitrine</strong> </td><td> <input type="text" name="plasmid2" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
| |
- | <tr><td><strong>pCMV_[PIR]_TAL-B:KRAB</strong> </td><td> <input type="text" name="plasmid3" size="5" value="190" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
| |
- | <tr><td><strong>pCMV_[ETR]_TAL-B:KRAB</strong> </td><td> <input type="text" name="plasmid4" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
| |
- | <tr><td><strong>pCMV_PIP:KRAB</strong> </td><td> <input type="text" name="plasmid5" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
| |
- | <tr><td><strong>pCMV_E:KRAB </strong> </td><td> <input type="text" name="plasmid6" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
| |
- | </table>
| |
- | <font color="red">(Enter dosages for corresponding plasmids.)</font><br /><br />
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- |
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- |
| |
- |
| |
- | <hr/>
| |
- | <b>TRANSCRIPTION FACTORS</b>
| |
- | <br />
| |
- | <input type="checkbox" name="req" value="1" /> Equal transcription factors
| |
- | <br/>
| |
- | <font color="red">(Transcription factors will alter transcription with equal rates)</font><br /> <br />
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- |
| |
- | <hr/>
| |
- | <b>INDUCERS</b> <br/>
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- |
| |
- | <table>
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- | <th><tr><td><i>Pristinamycin</i></td><td></td></tr></th>
| |
- | <tr>
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- | <td>Input times [min]</td>
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- | <td><input type="text" name="pc_start1" size="5" value="2000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
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- | <td><input type="text" name="pc_start2" size="5" value="10000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
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- | <td><input type="text" name="pc_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="pc_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
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- | <td><input type="text" name="pc_start5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
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- | </tr>
| |
- | <tr>
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- | <td>Durations [min]</td>
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- | <td><input type="text" name="pc_duration1" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
| |
- | <td><input type="text" name="pc_duration2" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
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- | <td><input type="text" name="pc_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
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- | <td><input type="text" name="pc_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
| |
- | <td><input type="text" name="pc_duration5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
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- | </tr>
| |
- | </table>
| |
- |
| |
- | <br/>
| |
- |
| |
- | <table>
| |
- | <th><tr><td><i>Erithromycin</i></td><td></td></tr></th>
| |
- | <tr>
| |
- | <td>Input times [min]</td>
| |
- | <td><input type="text" name="rg_start1" size="5" value="6000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="rg_start2" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="rg_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="rg_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="rg_start5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>Durations [min]</td>
| |
- | <td><input type="text" name="rg_duration1" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | <td><input type="text" name="rg_duration2" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | <td><input type="text" name="rg_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | <td><input type="text" name="rg_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | <td><input type="text" name="rg_duration5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | </tr>
| |
- | </table>
| |
- |
| |
- | <font color="red">(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )</font><br />
| |
- |
| |
- | <br />
| |
- | <b>SIMULATION</b> <br/>
| |
- |
| |
- |
| |
- | <hr />
| |
- | Time: <input type="text" name="time" size="5" value="15000" onKeyPress="return numbersonly(this, event)" maxlength="7"/> min<br />
| |
- | <font color="red">(Simulation running time)</font><br /><br />
| |
- |
| |
- | <input type="submit" value="SIMULATE" /> <br /><br /><br />
| |
- | </form>
| |
- | </body>
| |
- | </html>
| |
- |
| |
- | <html>
| |
- |
| |
- | <SCRIPT TYPE="text/javascript">
| |
- | <!--
| |
- | // copyright 1999 Idocs, Inc. http://www.idocs.com
| |
- | // Distribute this script freely but keep this notice in place
| |
- | function numbersonly(myfield, e, dec)
| |
- | {
| |
- | var key;
| |
- | var keychar;
| |
- |
| |
- | if (window.event)
| |
- | key = window.event.keyCode;
| |
- | else if (e)
| |
- | key = e.which;
| |
- | else
| |
- | return true;
| |
- | keychar = String.fromCharCode(key);
| |
- |
| |
- | // control keys
| |
- | if ((key==null) || (key==0) || (key==8) ||
| |
- | (key==9) || (key==13) || (key==27) )
| |
- | return true;
| |
- |
| |
- | // numbers
| |
- | else if ((("0123456789").indexOf(keychar) > -1))
| |
- | return true;
| |
- |
| |
- | // decimal point jump
| |
- | else if (dec && (keychar == "."))
| |
- | {
| |
- | myfield.form.elements[dec].focus();
| |
- | return false;
| |
- | }
| |
- | else
| |
- | return false;
| |
- | }
| |
- |
| |
- | //-->
| |
- | </SCRIPT>
| |
- |
| |
- | <body>
| |
- |
| |
- | <hr />
| |
- |
| |
- |
| |
- | <form name="form1" action="http://igem2012.fri.uni-lj.si:443/cellulator/bistable_feedback" target="_blank" method="GET">
| |
- | <input type="hidden" name="check_submit" value="1"/>
| |
- | <b>INPUT VECTORS DOSAGE</b>
| |
- |
| |
- | <table>
| |
- | <th>
| |
- | <tr><td>Construct</td><td>Dosage</td></tr>
| |
- | </th>
| |
- | <tr><td><strong>[A]_pCMV_TAL-B:KRAB_TAL-A:VP16_BFP </strong></td><td><input type="text" name="plasmid1" size="5" value="200"/> ng</td></tr>
| |
- | <tr><td><strong>[B]_pCMV_TAL-A:KRAB_TAL-B:VP16_mCitrine</strong></td><td><input type="text" name="plasmid2" size="5" value="200"/> ng</td></tr>
| |
- | <tr><td><strong>pCMV_[PIR]_TAL-B:KRAB_TAL-A:VP16</strong></td><td><input type="text" name="plasmid3" size="5" value="200"/> ng</td></tr>
| |
- | <tr><td><strong>pCMV_[ETR]_TAL-A:KRAB_TAL-B:VP16</strong></td><td><input type="text" name="plasmid4" size="5" value="200"/> ng</td></tr>
| |
- | <tr><td><strong>pCMV_PIP:KRAB_E:KRAB</strong></td><td><input type="text" name="plasmid5" size="5" value="200"/> ng</td></tr>
| |
- | </table>
| |
- | <font color="red">(Enter dosages for corresponding plasmids.)</font><br /><br />
| |
- |
| |
- | <hr/>
| |
- | <b>TRANSCRIPTION FACTORS</b>
| |
- | <br />
| |
- | <input type="checkbox" name="req" value="1" /> Equal transcription factors <br />
| |
- | <font color="red">(Transcription factors will alter transcription with equal rates)</font><br /><br />
| |
- | <hr/>
| |
- | <b>INDUCERS</b> <br/>
| |
- |
| |
- |
| |
- | <table>
| |
- | <th><tr><td><i>Pristinamycin</i></td><td></td></tr></th>
| |
- | <tr>
| |
- | <td>Input times [min]</td>
| |
- | <td><input type="text" name="pc_start1" size="5" value="7000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="pc_start2" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="pc_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="pc_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | <td><input type="text" name="pc_start5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>Durations [min]</td>
| |
- | <td><input type="text" name="pc_duration1" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
| |
- | <td><input type="text" name="pc_duration2" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
| |
- | <td><input type="text" name="pc_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
| |
- | <td><input type="text" name="pc_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
| |
- | <td><input type="text" name="pc_duration5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
| |
- | </tr>
| |
- | </table>
| |
- |
| |
- | <br/>
| |
- |
| |
- | <table>
| |
- | <th><tr><td><i>Erithromycin</i></td><td></td></tr></th>
| |
- | <tr>
| |
- | <td>Input times [min]</td>
| |
- | <td><input type="text" name="rm_start1" size="5" value="3000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="rm_start2" size="5" value="14000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="rm_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="rm_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | <td><input type="text" name="rm_start5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
| |
- | </tr>
| |
- | <tr>
| |
- | <td>Durations [min]</td>
| |
- | <td><input type="text" name="rm_duration1" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | <td><input type="text" name="rm_duration2" size="5" value="2000" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | <td><input type="text" name="rm_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | <td><input type="text" name="rm_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | <td><input type="text" name="rm_duration5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
| |
- | </tr>
| |
- | </table>
| |
- | <font color="red">(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )</font>
| |
- | <br />
| |
- | <br />
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | <hr/>
| |
- | <b>SIMULATION</b> <br/>
| |
- | Time: <input type="text" name="time" size="5" value="20000"/> min<br />
| |
- | <font color="red">(Simulation running time)</font><br />
| |
- | <br />
| |
- |
| |
- | <input type="submit" value="SIMULATE" />
| |
- | </form>
| |
- | </body>
| |
- | </html>
| |
- |
| |
- |
| |
- | <!--
| |
- | == Tristable switch (2 inputs) ==
| |
- | == Tristable switch with positive feedback (2 inputs) ==
| |
- | -->
| |