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Team:Columbia-Cooper-NYC/Primers
From 2012.igem.org
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= Preparation of Primer Stock Protocol = | = Preparation of Primer Stock Protocol = | ||
| + | * Protocol for preparing the concentrated primers to be able to be used in DNA sequencing is the following: | ||
*# Add 20x primer weight of deionized water to concentrated primer | *# Add 20x primer weight of deionized water to concentrated primer | ||
*# Vortex 20 seconds of sample with concentrated primer and deionized water | *# Vortex 20 seconds of sample with concentrated primer and deionized water | ||
*# Centrifuge for 10 seconds of the sample | *# Centrifuge for 10 seconds of the sample | ||
*# Diluted sample 100x again for use as stock | *# Diluted sample 100x again for use as stock | ||
Revision as of 15:38, 22 September 2012
Preparation of Primer Stock Protocol
- Protocol for preparing the concentrated primers to be able to be used in DNA sequencing is the following:
- Add 20x primer weight of deionized water to concentrated primer
- Vortex 20 seconds of sample with concentrated primer and deionized water
- Centrifuge for 10 seconds of the sample
- Diluted sample 100x again for use as stock
