Project

The idea is a continuation of the EPFL's iGEM 2009 project. The initial plasmid designs come from a team member of the 2009 team. The cloning and troubleshooting and other further developments of these plasmids were done by the current team.

Experiments

The protocols mostly come from our advisors, but also from the kit suppliers and from the relevant core facilities at the EPFL. The advisors helped us learn the basics for most experiments. The cloning and the work in the mammalian cell lab was done by the team. The experimental designs come from the team and were reviewed by our advisors.

The DH5alpha host strain, CHO DG44 and HEK cells were kindly provided to use by the LBTC, the lab who hosted us for this summer.

Some flowcytometry and most microscopy experiments were performed by core facilities at the EPFL (as the devices we needed can't be used without a full training course).

Graphics & Website

All graphic design was done by the team. For the website, some open-source libraries/tools were used (Bootstrap, jQuery, Coffeescript) to help the development process.