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<!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> | ||
| - | Download the following spreadsheet : [[File:Team-EPF-Lausanne Ligation.xls]] | + | # Download the following spreadsheet : [[File:Team-EPF-Lausanne Ligation.xls]] |
| - | Fill in the pink areas with the vector and fragment concentration, their size and the ratio. | + | # Fill in the pink areas with the vector and fragment concentration, their size and the ratio. |
| + | |||
| + | # Add all the suggested ingradients in a microcentrifuge tube, in the order they appear. | ||
| + | |||
| + | # Ligate for 2 hours at 14ºC. | ||
| + | |||
| + | # Inmediatly transform competent bacteria with the ligation product. | ||
{{:Team:EPF-Lausanne/Template/ProtocolFooter}} | {{:Team:EPF-Lausanne/Template/ProtocolFooter}} | ||
<noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | <noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | ||
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