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| - | NOTE: This is a template for entering things for the time being. All dates should be enclosed in <h2> tags and all paragraphs should be enclosed in <p> tags. For bulleted lists, <ul> tags will create the list and <li> tags will surround each list item. If there are any questions, please let me know.
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| - | Patrick.
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| - | <h2>Week 1 (May 1-4)</h2>
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| - | <p>This was the first week where we met with other team members and summarized the primary subprojects the team will be tackling this coming summer.</p>
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| - | <h2>Week 2 (May 7-11)</h2>
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| - | <p> During this week literature search was performed.</p>
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| - | <h2>Week 3 (May 14-18)</h2>
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| - | <p> During this week literature search was performed.</p>
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| - | <h2>Week 4 (May 22-25)</h2>
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| - | <p> During this week, strains of <i> Pseudomonas fluorescens</i> PF-5 were obtained. Two cultures were started by adding 500 µL stock to 10 mL LB media containing 50 mg/L ACROS Napthenic Acids. These cultures were grown at 30°C overnight, shaking at 110 rpm.</p>
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| - | <p> Overnight cultures were then plated on LB agar the following day on various types and concentrations of antibiotics in order to determine the susceptibility profile. This was necessary in order to determine what selectable marker could be used on a transposon. These plates were grown overnight, and the following results were obtained; </p>
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| - | <p><center><b>Gentamycin</b></p>
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| - | <p>25 µg/ml = no growth</p>
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| - | <p>50 µg/ml = no growth</p>
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| - | <p>100 µg/ml = no growth</p>
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| - | <p>
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| - | <p><b>Kanamycin</b></p>
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| - | <p>5 µg/ml = slight growth</p>
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| - | <p>10 µg/ml = slight growth</p>
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| - | <p>25 µg/ml = no growth</p>
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| - | <p>50 µg/ml = no growth</p>
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| - | <p><b>Chloramphenicol</b></p>
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| - | <p>5 µg/ml = growth</p>
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| - | <p>10 µg/ml = growth</p>
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| - | <p>25 µg/ml = growth</p>
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| - | <p>50 µg/ml = growth</p>
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| - | <p>
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| - | <p><b>Tetracycline</b></p>
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| - | <p>50 µg/ml = no growth</p>
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| - | <p>100 µg/ml = no growth</p>
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| - | <p>200 µg/ml = no growth</center></p>
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| - | <p>
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| - | <p>Based on these results, it was determined that Kanamycin, Gentamycin, and Tetracycline could be used as the selectable marker on the transposon, while Chloramphenicol could not as the strain is naturally resistant. Glycerol stocks of the strains were also made at this time from a fresh overnight culture.</p>
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| - | <h2>Week 5 (May 28-June 1)</h2>
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