Team:Austin Texas/Plasmids and Strains

From 2012.igem.org

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| Barrick Lab
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| iGEM 2012 Box 1
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| IGM009
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| crdASC (4864 bp) in TOP10
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| Barrick Lab
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| iGEM 2012 Box 1
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| 8
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| IGM010
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| crdASC (4943 bp) in TOP10
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| Barrick Lab
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| iGEM 2012 Box 1
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| 9
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| IGM011
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| crdASC (4864 bp) in BL21(DE3)
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| Barrick Lab
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| iGEM 2012 Box 1
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| 10
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| IGM012
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| crdASC (4943 bp) in BL21(DE3)
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| Barrick Lab
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Revision as of 00:05, 18 June 2012

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box Slot Label Name Part Plasmid Source
iGEM 2012 Plasmid Box 1 2 cadA ASKA cadA plasmid Barrick Lab
iGEM 2012 Plasmid Box 1 3 ldcC ASKA ldcC plasmid Barrick Lab
iGEM 2012 Plasmid Box 1 IGM004 GFP [http://www.partsregistry.org/Part:BBa_I714891 BBa_I714891] pSB3K3 2012 kit plate 2 (15L)
iGEM 2012 Plasmid Box 1 IGM005 RFP [http://www.partsregistry.org/Part:BBa_K081014 BBa_K081014] pSB1AK3 2012 kit plate 3 (12O)
iGEM 2012 Plasmid Box 1 IGM006 LasI [http://www.partsregistry.org/Part:BBa_K081009 BBa_K081009] pSB1A2 2012 kit plate 2 (10N)
iGEM 2012 Plasmid Box 1 IGM007 RBS + LacI [http://www.partsregistry.org/Part:BBa_J24679 BBa_J24679] pSB1A2 2012 kit plate 1 (7L)
iGEM 2012 Plasmid Box 1 IGM008 Double terminator (BBa_B0010-BBa_B0012) [http://www.partsregistry.org/Part:BBa_B0015 BBa_B0015] pSB1AK3 2012 kit plate 1 (23L)

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box Slot Name Description Source
iGEM 2012 Box 1 2 AG1 Host strain of ASKA collection Barrick Lab
iGEM 2012 Box 1 3 BW25113 Host strain of Keio collection Barrick Lab
iGEM 2012 Box 1 4 cadA AG1 + ASKA collection cadA plasmid, CamR Barrick Lab
iGEM 2012 Box 1 5 ldcC AG1 + ASKA collection ldcC plasmid, CamR Barrick Lab
iGEM 2012 Box 1 6 tnaA Keio collection knockout (version of odor-less E. coli), KanR Barrick Lab
iGEM 2012 Box 1 7 IGM009 crdASC (4864 bp) in TOP10 Barrick Lab
iGEM 2012 Box 1 8 IGM010 crdASC (4943 bp) in TOP10 Barrick Lab
iGEM 2012 Box 1 9 IGM011 crdASC (4864 bp) in BL21(DE3) Barrick Lab
iGEM 2012 Box 1 10 IGM012 crdASC (4943 bp) in BL21(DE3) Barrick Lab

ZombiE.coli parts

Plasmid Part Description Source
pReporter [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) 2012 kit plate 2 (15L)
pReporter [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] RFP protein generator - PoPS->RFP 2012 kit plate 3 (12O)
pReporter [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] LasI protein generator (TERM-) 2012 kit plate 2 (10N)
pReporter [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] RBS + LacI 2012 kit plate 1 (7L)
pReporter [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Double terminator (BBa_B0010-BBa_B0012) 2012 kit plate 1 (23L)
pConverter [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] LasR Promoter 2012 kit plate 1 (12A)
pConverter [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] LasR 2012 kit plate 1 (14J)
pConverter [http://partsregistry.org/Part:BBa_K082016 BBa_K082016] LacIM1 Parts Registry
pConverter [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] LacI Promoter 2012 kit plate 1 (1D)
pConverter CRE pQL269 Plasmid
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