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Team:Frankfurt/Organisms
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''S.cerevisiae'' is our main organism. We want to produce steviol in yeast and we did the transformations in this organism. In addition we are able to do the transformations via gap repair which is possible in yeast because of its ability for homologue recombination with only about 40 bp. Furthermore ''S.cerevisiae'' has got an ER which is needed because some enzymes for steviol production are localized in the ER, ''S.cerevisiae'' is an established food additive producer and is closer related to ''Stevia'' than ''E.coli'' for example. | ''S.cerevisiae'' is our main organism. We want to produce steviol in yeast and we did the transformations in this organism. In addition we are able to do the transformations via gap repair which is possible in yeast because of its ability for homologue recombination with only about 40 bp. Furthermore ''S.cerevisiae'' has got an ER which is needed because some enzymes for steviol production are localized in the ER, ''S.cerevisiae'' is an established food additive producer and is closer related to ''Stevia'' than ''E.coli'' for example. | ||
| - | ==Organisms from which | + | ==Organisms from which genes were derived== |
===''Sulfolobus acileratius''=== | ===''Sulfolobus acileratius''=== | ||
We used the GGPP synthase from this organism. | We used the GGPP synthase from this organism. | ||
Revision as of 08:22, 21 September 2012
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Organisms
Organisms used in our LabWork
Escherichia coli
E.coli is a well known model organism for molecular biology. It is a gram negative bacteria whose generation time is about 30 minutes. We used E.coli to amplify the plasmids that we isolated from yeast after transformation via homologue recombination.
Saccharomyces cerevisiae
S.cerevisiae is our main organism. We want to produce steviol in yeast and we did the transformations in this organism. In addition we are able to do the transformations via gap repair which is possible in yeast because of its ability for homologue recombination with only about 40 bp. Furthermore S.cerevisiae has got an ER which is needed because some enzymes for steviol production are localized in the ER, S.cerevisiae is an established food additive producer and is closer related to Stevia than E.coli for example.
Organisms from which genes were derived
Sulfolobus acileratius
We used the GGPP synthase from this organism.
Gibberella fujikuroi
We used the kaurene oxidase from this organism.
