Team:Goettingen/Test

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{{Team:Goettingen/Header}}
{{Team:Goettingen/Header}}
 +

Revision as of 13:01, 20 September 2012

{{Team:Goettingen/Header}}


Language: English, Deutsch

Contents

[hide]

Welcome to iGEM Göttingen

iGEM is an international competition hosted by the MIT in Boston, USA, for undergraduate students of disciplines related to molecular biology. iGEM stands for International Genetically Engineered Machine competition. On the one hand, it targets to combine aspects of education and social collaboration among undergraduate students, on the other hand, it provides a library for standardized and interchangeable parts which can be used in living systems, particularly in model organisms like E. coli. Student groups from all over the world will receive a requested kit of biological parts, also called "biobricks", and work over the summer on an individual research project. Every year, the "Registry of Standard Biological Parts" is upgraded with further biobricks by the participating iGEM teams. These biobricks will be accessible to the iGEM community in the following years in order to use these parts for their own projects.

The iGEM competition started in 2003 as a course for students of the MIT only. 2011, already 165 universities from all over the world competed against each other. This year, it is the first participation of the University of Göttingen, Germany. The iGEM competition is a great opportunity for students to gain and improve a multitude of skills that are necessary during a carrier as a molecular biologist. Such skills would be planning and organizing a project, including fund raising and team recruitment, coordination of lab work, designing experiments and working together as a team. Next to intensive lab work during the summer, also literature search and well-structured documentation of the experiments is crucial. Moreover, safety for humans and environment is key.


Homing Coli

Escherichia coli is a commonly used bacterial model organism. It has lots of beneficial traits, e.g. a short generation time and it can be easily manipulated. Most E. coli strains that are used in laboratories do not exhibit high motility. The crucial element for motility is the flagellum, which is rotated by a molecular motor within the cell wall. Consequently, these are reduced in cultivated E. coli strains.

Our goal is to create an E. coli strain with increased swimming motility on special agar plates. Therefore, we will overexpress regulators and parts of the E. coli flagellum in cultivated strains in order to enhance bacterial swimming ability. The fastest E. coli strains will be selected and further improved. At the same time we will then be able to create an effective motility-selection method.

Now, you are probably wondering what the advantage of a fast E. coli might be. The beneficial fast phenotype can be combined with the ability of this bacterium to sense specific compounds in their environments. Chemoreceptors enable it to move towards or along gradients of such substances. We will use PCR-based site directed mutagenesis on the E. coli TAR receptor. The combination of speed and chemotaxis allows us to identify E. coli strains, which can sense interesting compounds. Thereby, an easy method for the detection of pollutants, toxins or even tumors could be provided.

Clive S. Barker et al. (2004). Increased Motility of Escherichia coli by Insertion Sequence Element Integration into the Regulatory Region of the flhD Operon. Journal of Bacteriology, Vol. 186: 7529-7537.


Synthetic Biology

Synthetic biology is an interdisciplinary scientific area that has recently developed. It links various fields of science like biology, chemistry, physics, molecular genetics, informatics and engineering.

Due to this combination the relation of biological design and function can be investigated from an entirely new perspective. The previous approach was limited to the examination of a structure and its function and the attempt to explain how they correlate. Recently, the reverse strategy is applied. Biological parts are specifically designed and constructed according to a desired function. These parts are characterized by a standardized modular design that facilitates their handling. The subsequent introduction of the synthetic constructs to living cells can either cause the replacement of original cellular components or result in additional elements that act cooperatively or more or less autonomously (see fig. 1).


After Nandagopaland and Elowitz (2011). A continuum of synthetic biology. Wild-type cells (A) can be subject to two basic types of synthetic manipulation. (B) Autonomous synthetic circuits, consisting of ectopic components, may be introduced into the cell. Such circuits process inputs and implement functions (red arrows) seperate from the endogenous circuitry (black). However, unknown interactions with the host cell may affect their function (purple arrows) (C)An alternative is to rewire (red lines) the endogenous circuits themselves to have new connectivity. (D) Extending this line of synthetic manipulation, synthetic circuits could be integrated into appropriately rewired endogenous circuitry to act as sensors and to implent additional functionality. Ultimate goals of this program are to be able to design and construct (E) synthetic circuits that can functionality replace endogenous circuits or (F) fully autonomous circuits that operate independently of the cellular mileu. Nagarajan Nandagopal and Michael B. Elowitz. (2011). Synthetic Biology: Integrated Gene Circuits. SCIENCE, Vol. 333: 1244-1248.

Another very important and necessary feature of biological parts is orthogonality, which means in this context that independent devices can be combined unrestrictedly. This principle derives from the area of engineering and aims to alter subsystems, without impeding others.

This way cells can be modified as requested, resulting in a predictable behaviour. Among others, this technique can be beneficial for the effective production of certain substances, like biofuels or antibiotics.



↑ Return to top

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

Flash Coli

To explore our project with Flash Coli click here.


News


07 Septembre 2012, We organized the "Legoland for Scientists" symposium, at the University of Goettingen. You can read everything here

To the news

30 August 2012, The first version of 'Flash Coli - the game' is online now. click here

To the news

25 August 2012, Today is the "synthetic biology" day in Germany!

To the news

15 August 2012, In our weekly seminar Anna presented an example for synthetic Biology

To the news

08 August 2012, Ali and Sandra conducted their presentations in our weekly seminar

To the news

12 July 2012, In today’s weekly meeting Christian told us about the European teacher meet-up in Paris

To the news

28 June 2012, Anna, Corinna and Erik participated in the third annual congress for strategy processes ”Biotechnology 2020+”

To the news

21 June 2012, Our first poster is designed

To the news

06 June 2012, Thomas Maschner from LMU Munich came to visit

To the news

15 May 2012, The first picture of our complete Team

To the news

11 May 2012, iGEM Göttingen is in the local Newspaper

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04 May 2012, KWS SAAT AG is now Head Sponsor

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02 May 2012, Today Anna presented our iGEM Logo

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30 April 2012, We inspect the Laboratory at the Max Planck Campus

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26 April 2012, Weekly meeting: PCR-Lecture from Heinz

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19 April 2012, The iGEM Team Göttingen 2012 is now complete!

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04 April 2012, Sartorius Stedim Biotech GmbH is now Premium Sponsor

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