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Revision as of 13:53, 19 September 2012
Chemically Defined Sporulation Media (CDSM)
ref.: W. Nicholson & P. Setlow, in Molecular Biological Methods for Bacillus, eds. C. Harwood & S. Cutting, New York: John Wiley, section 9, 1990.
CDSM
Notes: 120mL CDSM makes one batch of spores Adujst stock solutions for each below to pH7.0 with KOH (except for MT mix). Autoclave stock solutions, except MOPS and L-Glutamic acid which are filter sterilised. L-Glutamic acid is made up in 1M HCl
- 40mM morpholinopropanesulfonic acid (MOPS)
- 4.0mM KH2PO4
- 9.5mM (NH4)2SO4
- 5.0mM L-lactic acid
- 8.0mM L-Glutamic acid
- 20mM - D-Glucose
- 1xMT mix
50xMT mix (trace metal mix)
Note: Add FeCl3 last to avoid precipitation This solutions makes 50L CDSM
For 1L:
- 2ml 1M HCl
- 40.6g Mg Cl2
- 10.29g CaCl2
- 0.99g MnCl2
- 13.6g ZnCl2
- 67.5mg Thiamine-HCL
- 135mg FeCl3
