Team:WashU/Protocols/PCR

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=General PCR Protocol=
=General PCR Protocol=

Revision as of 20:26, 13 June 2012


General PCR Protocol

  1. Set up PCR reaction tube
    1. 1uL Template
    2. 1uL 25mM dNTP's
    3. 10uL Phusion HF Buffer
    4. 0.5uL Forward Primer
    5. 0.5uL Reverse Primer
    6. 0.5uL TAQ polymerase
    7. 36.5uL diH2O
  1. Amplification reaction in thermocycler
    1. 1 cycle 94 degrees (3 minutes)
    2. 40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
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