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Team:WashU/Protocols/PCR
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(Difference between revisions)
(Created page with "=General PCR Protocol= #Setup Amplification PCR Reaction ##1uL Template ##1uL 25mM dNTP's ##10uL Phusion HF Buffer ##0.5uL Forward Primer (Tm XX <sup>o</sup>C)* ##0.5uL Reverse ...") |
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=General PCR Protocol= | =General PCR Protocol= | ||
| - | # | + | #Set up PCR reaction tube |
##1uL Template | ##1uL Template | ||
##1uL 25mM dNTP's | ##1uL 25mM dNTP's | ||
| Line 7: | Line 7: | ||
##0.5uL Forward Primer (Tm XX <sup>o</sup>C)* | ##0.5uL Forward Primer (Tm XX <sup>o</sup>C)* | ||
##0.5uL Reverse Primer (Tm YY <sup>o</sup>C)* | ##0.5uL Reverse Primer (Tm YY <sup>o</sup>C)* | ||
| - | ##0.5uL | + | ##0.5uL TAQ polymerase |
##36.5uL diH2O | ##36.5uL diH2O | ||
| + | |||
| + | #Amplification reaction in thermocycler depends | ||
Revision as of 20:22, 13 June 2012
General PCR Protocol
- Set up PCR reaction tube
- 1uL Template
- 1uL 25mM dNTP's
- 10uL Phusion HF Buffer
- 0.5uL Forward Primer (Tm XX oC)*
- 0.5uL Reverse Primer (Tm YY oC)*
- 0.5uL TAQ polymerase
- 36.5uL diH2O
- Amplification reaction in thermocycler depends
