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| | <noinclude>{{:Team:EPF-Lausanne/Template/Header}}</noinclude> | | <noinclude>{{:Team:EPF-Lausanne/Template/Header}}</noinclude> |
| - | {{:Team:EPF-Lausanne/Template/ProtocolHeader|DNA concentration measurement|{{{1|}}}}} | + | |
| | + | <noinclude>{{:Team:EPF-Lausanne/Template/SetTitle|Nanodrop}}</noinclude> |
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| | + | {{:Team:EPF-Lausanne/Template/ProtocolHeader|DNA Concentration Measurement|{{{1|}}}}} |
| | <!-- /\ --> | | <!-- /\ --> |
| | <!-- || --> | | <!-- || --> |
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| | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> | | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> |
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| - | * Take a new 6 µl aliquote of the DNA (under the hood) and put back the main DNA tube in the fridge. | + | * Take a 6 µl aliquote of the DNA and put back the main DNA tube in the fridge. |
| - | * Go to the room by the E. coli lab (not on Friday morning!) with: | + | * Go to the room by the E.Coli lab (LBTM, not on Friday morning!) with: |
| | ** The 6 µl aliquote | | ** The 6 µl aliquote |
| - | ** A 10 µl pipete | + | ** A 10 µl pipet |
| - | ** Eventually some 5 µl of TE buffer (they migh have some). | + | ** Optionally, the buffer you used for DNA elution (there might be some next to the machine). |
| | * The machine is the NanoDrop Spectrophotometer. | | * The machine is the NanoDrop Spectrophotometer. |
| | * On the computer, click on "Nucleic Acid". | | * On the computer, click on "Nucleic Acid". |
| - | * Add 2 µl of (nuclease free) water to the machine's tip as you are asked to and measure. | + | * Put a 2 µl drop of (nuclease-free) water on the machine's tip as you are asked to and measure. |
| - | * Clean tips (both sides) with a tissue. | + | * Clean tips (both sides) with a quarter of tissue. |
| - | * Add 2 µl of TE buffer and click on "Blank". | + | * Add 2 µl of the buffer you use and click on "Blank". |
| - | * Clean tips (both sides) with a tissue. | + | * Clean tips (both sides). |
| - | * Add 2 µl of DNA and click "Measure". | + | * Add 2 µl of your DNA sample and click "Measure". |
| | * Clean tips (both sides) with a tissue. | | * Clean tips (both sides) with a tissue. |
| - | * Take 2 measurements per sample and average. | + | * Take 2 measurements per sample (for averaging). |
| | + | * Print the report when you are done |
| | * Click on exit. | | * Click on exit. |
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