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| | <noinclude>{{:Team:EPF-Lausanne/Template/Header}}</noinclude> | | <noinclude>{{:Team:EPF-Lausanne/Template/Header}}</noinclude> |
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| - | {{:Team:EPF-Lausanne/Template/SetTitle|Sampling for Tecan machine}} | + | <noinclude>{{:Team:EPF-Lausanne/Template/SetTitle|Sampling for Tecan Machine}}</noinclude> |
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| - | {{:Team:EPF-Lausanne/Template/ProtocolHeader|Sampling for Tecan machine|{{{1|}}}}} | + | {{:Team:EPF-Lausanne/Template/ProtocolHeader|Sampling for Tecan Machine|{{{1|}}}}} |
| | <!-- /\ --> | | <!-- /\ --> |
| | <!-- || --> | | <!-- || --> |
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| | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> | | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> |
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| - | Download the following spreadsheet : [[File:Team-EPF-Lausanne Ligation.xls]]
| + | The Tecan plate reader is used to measure fluorescence. It is useful if you want to quantify your fluorescent readout relatively to a baseline. |
| | + | To control it, use the provided Excel utility. |
| | + | |
| | + | 1. Take ~1 million cells in 750 µL PBS. |
| | + | |
| | + | 2. Centrifuge at 450g for 5 min. |
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| | + | 3. Remove the supernatant. |
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| | + | 4. Resuspend in 200µL of 1% TritonX in PBS. |
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| | + | 5. Load onto a black well plate and leave in the shaker for 1h. |
| | + | |
| | + | 6. Read fluorescence on the machine using the appropriate Excel program. |
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| - | Fill in the pink areas with the vector and fragment concentration, their size and the ratio.
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| | {{:Team:EPF-Lausanne/Template/ProtocolFooter}} | | {{:Team:EPF-Lausanne/Template/ProtocolFooter}} |
| | <noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | | <noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> |
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