Team:EPF-Lausanne/Protocol/PreparePlasmidExtraction

(Difference between revisions)

Latest revision as of 00:30, 18 September 2012

Select and number colonies on the plates.
Prepare tubes of LB medium with the correct quantity of antibiotics (100 µg/ml for Amp, Spc or chloramphenicol).
- Amp can be found in the -20ºC freezer at Ecoli, labeled as "stock". It is 100 µg/µl, or 1000x.
- The tubes to be used are the 14 ml round bottom found in front of the iGEM drawers (Falcon). Culture with cap in the first step (loose) and close to the second step after culture.
Touch each colony with a clean pipette tip and put it in a tube.
Put in incubator.

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+<noinclude>{{:Team:EPF-Lausanne/Template/SetTitle|Culture for Plasmid Extraction}}</noinclude>
+{{:Team:EPF-Lausanne/Template/ProtocolHeader|Prepare Plasmid Extraction (culture for Miniprep)|{{{1|}}}}}
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 * Select and number colonies on the plates.
-* Prepare tubes of ~3ml of LB medium with the correct quantity of antibiotics (100ug/ml for Amp and Spc).
+* Prepare tubes of LB medium with the correct quantity of antibiotics (100 µg/ml for Amp, Spc or chloramphenicol).
+** Amp can be found in the -20ºC freezer at Ecoli, labeled as "stock". It is 100 µg/µl, or 1000x.
+** The tubes to be used are the 14 ml round bottom found in front of the iGEM drawers (Falcon). Culture with cap in the first step (loose) and close to the second step after culture.
 * Touch each colony with a clean pipette tip and put it in a tube.
 * Put in incubator.