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| | <noinclude>{{:Team:EPF-Lausanne/Template/Header}}</noinclude> | | <noinclude>{{:Team:EPF-Lausanne/Template/Header}}</noinclude> |
| - | {{:Team:EPF-Lausanne/Template/ProtocolHeader|Ampicillin Agar Plates|{{{1|}}}}} | + | |
| | + | <noinclude>{{:Team:EPF-Lausanne/Template/SetTitle|Plates with Antibiotic}}</noinclude> |
| | + | |
| | + | {{:Team:EPF-Lausanne/Template/ProtocolHeader|Agar Plates|{{{1|}}}}} |
| | <!-- /\ --> | | <!-- /\ --> |
| | <!-- || --> | | <!-- || --> |
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| | ** 20 g/l of LB broth powder. | | ** 20 g/l of LB broth powder. |
| | ** 10 g/l of Agar (not agarose). | | ** 10 g/l of Agar (not agarose). |
| - | * Fill up with di water and autoclave (program 106, takes 2 hours). Remember to leave the cap loose! | + | * Fill up with DI water and autoclave (program 106, takes 2 hours). Remember to leave the cap loose! |
| | * Label the plates (found in the stock room). | | * Label the plates (found in the stock room). |
| - | * When autoclave is ready, close the bottle cap and take the bottle out. | + | * When the autoclaving is done, close the bottle cap and take the bottle out. |
| - | * Let rest until it cools down to around 55ºC (can be hold for some seconds): if warmer, the antibioticum degrades, if colder the broth gelifies. | + | * Let rest until it cools down to around 55ºC (can be held for some seconds): if warmer, the antibiotic degrades, if colder, the broth gelifies. |
| - | * Add the antibioticum (Ampicillin 100 ug/ml) = 1 ml of 100 mg/ml Amp for 1 l of broth. | + | * Add the antibiotic (Ampicillin 100 µg/ml) = 1 ml of 100 mg/ml Amp for 1l of broth. Same for chloramphenicol or Spe. |
| | * Add 25-30 ml of broth to each plate and let open for 2 hours. | | * Add 25-30 ml of broth to each plate and let open for 2 hours. |
| - | * Close plates, wrap in alu foil (amp sensitive to light) and store at +4ºC. | + | * Close plates, wrap in alu foil (Amp is sensitive to light) and store at +4ºC. |
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| | {{:Team:EPF-Lausanne/Template/ProtocolFooter}} | | {{:Team:EPF-Lausanne/Template/ProtocolFooter}} |
| | <noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | | <noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> |
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