Protocol: TAE
To make 200 ml of %0x TAE :
- Tris base: 78.4 g
- Acetic Acid (100%): 11.4 ml
- EDTA: 20ml 0.5 M
- 292.25M --> 146.12 = 0.5 mol --> 1.46 g in 10 ml is 0.5M
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- | To make | + | To make 200 ml of %0x TAE : |
- | * Tris base : 78.4 g | + | * Tris base: 78.4 g |
- | * Acetic Acid (100 | + | * Acetic Acid (100%): 11.4 ml |
- | * EDTA : 20ml 0.5 M | + | * EDTA: 20ml 0.5 M |
** 292.25M --> 146.12 = 0.5 mol --> 1.46 g in 10 ml is 0.5M | ** 292.25M --> 146.12 = 0.5 mol --> 1.46 g in 10 ml is 0.5M | ||
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To make 200 ml of %0x TAE :