Team:EPF-Lausanne/Protocol/TAE

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To make a new 200 ml of %0x TAE :
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To make 200 ml of %0x TAE :
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* Tris base : 78.4 g
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* Tris base: 78.4 g
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* Acetic Acid (100 %) : 11.4 ml
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* Acetic Acid (100%): 11.4 ml
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* EDTA : 20ml 0.5 M
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* EDTA: 20ml 0.5 M
** 292.25M --> 146.12 = 0.5 mol --> 1.46 g in 10 ml is 0.5M
** 292.25M --> 146.12 = 0.5 mol --> 1.46 g in 10 ml is 0.5M
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Latest revision as of 00:28, 18 September 2012

Protocol: TAE


To make 200 ml of %0x TAE :

  • Tris base: 78.4 g
  • Acetic Acid (100%): 11.4 ml
  • EDTA: 20ml 0.5 M
    • 292.25M --> 146.12 = 0.5 mol --> 1.46 g in 10 ml is 0.5M