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Team:Wageningen UR/Journal/week17
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== Lab work == | == Lab work == | ||
| + | '''Hepatitis B''' | ||
| + | |||
| + | ---- | ||
| + | ''20 August'' | ||
| + | * Colony PCR | ||
| + | - of HepB (without promoter) in BBa_J04450 | ||
| + | - of HepB with a his tag + IPTG promoter in Bba_J04500 | ||
| + | |||
| + | * Ligation | ||
| + | - of the HepB with the IPTG promoter into BBa_J04450 | ||
| + | |||
| + | * Transformation | ||
| + | Both into JM109 and DH5α; using the BBa_J04450 brick as positive control | ||
| + | |||
'''Hepatitis B inside modification''' | '''Hepatitis B inside modification''' | ||
---- | ---- | ||
| + | |||
| + | |||
| + | |||
| + | '''GFP modification''' | ||
| + | |||
| + | ---- | ||
| + | |||
| + | ''20 August'' | ||
| + | * Ligation | ||
| + | - of the digested GFP-coil PCR product from 15.Aug into BBa_J04450 | ||
| + | |||
| + | * Transformation | ||
| + | Both into JM109 and DH5α; using the BBa_J04450 brick as positive control | ||
Revision as of 09:51, 17 September 2012
week 17: 20 august - 26 august
Office work
Lab work
Hepatitis B
20 August
- Colony PCR
- of HepB (without promoter) in BBa_J04450 - of HepB with a his tag + IPTG promoter in Bba_J04500
- Ligation
- of the HepB with the IPTG promoter into BBa_J04450
- Transformation
Both into JM109 and DH5α; using the BBa_J04450 brick as positive control
Hepatitis B inside modification
GFP modification
20 August
- Ligation
- of the digested GFP-coil PCR product from 15.Aug into BBa_J04450
- Transformation
Both into JM109 and DH5α; using the BBa_J04450 brick as positive control
