Team:Macquarie Australia/Protocols/GibsonAssembly2

From 2012.igem.org

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<td>1.addition of 20ng gene fragments in appropriate tube</td>
<td>1.addition of 20ng gene fragments in appropriate tube</td>
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<td>2μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E </td>
+
<td>2 μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E </td>
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<td>2μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E </td>
+
<td>2 μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E </td>
-
<td>2μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E </td>
+
<td>2 μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E </td>
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<td>2μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
+
<td>2 μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
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<td>2μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
+
<td>2 μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
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<td>2μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
+
<td>2 μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
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<td>2μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
+
<td>2 μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
-
<td>2μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
+
<td>2 μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
-
<td>2μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
+
<td>2 μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E</td>
</tr>
</tr>
<tr>
<tr>
<td>2. Addition of 0.05pmol of vector</td>
<td>2. Addition of 0.05pmol of vector</td>
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<td>2.8µl PSB-1A3</td>
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<td>2.8 µl PSB-1A3</td>
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<td>2.9µl PSB-1K3</td>
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<td>2.9 µl PSB-1K3</td>
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<td>2.7µl PSB-1C3</td>
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<td>2.7 µl PSB-1C3</td>
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<td>2.8µl PSB-1A3</td>
+
<td>2.8 µl PSB-1A3</td>
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<td>2.9µl PSB-1K3</td>
+
<td>2.9 µl PSB-1K3</td>
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<td>2.7µl PSB-1C3</td>
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<td>2.7 µl PSB-1C3</td>
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<td>2.8µl PSB-1A3</td>
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<td>2.8 µl PSB-1A3</td>
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<td>2.9µl PSB-1K3</td>
+
<td>2.9 µl PSB-1K3</td>
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<td>2.7µl PSB-1C3</td>
+
<td>2.7 µl PSB-1C3</td>
</tr>
</tr>
<tr>
<tr>

Revision as of 07:24, 17 September 2012



3A 3K 3C 4A 4K 4C 5A 5K 5C
1.addition of 20ng gene fragments in appropriate tube 2 μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E 2 μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E 2 μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E 2 μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E 2 μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E 2 μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E 2 μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E 2 μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E 2 μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E
2. Addition of 0.05pmol of vector 2.8 µl PSB-1A3 2.9 µl PSB-1K3 2.7 µl PSB-1C3 2.8 µl PSB-1A3 2.9 µl PSB-1K3 2.7 µl PSB-1C3 2.8 µl PSB-1A3 2.9 µl PSB-1K3 2.7 µl PSB-1C3
3. Addition of Master Mix (μl) 12.8 12.9 12.7 12.8 12.9 12.7 12.8 12.9 12.7

Retrieved from "http://2012.igem.org/Team:Macquarie_Australia/Protocols/GibsonAssembly2"