Team:Penn/Notebook

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Revision as of 10:32, 15 September 2012

Penn 2012 iGEM Wiki

June 2012 Notebook

Week 1

June 6th

  • Set up some lab equipment
  • Autoclaved for a while
  • Organized biobrick stuff
  • Called Vinoo about DNA planning

June 7th

  • Transformed Cph8, pLsr, and LuxS
  • Placed order with Vinoo
  • Developed idea using PGY/PCN system to activate a gene

Week 2

June 11th

  

Wet Lab

  • PCR'd mCherry from NAS157
  • Ran 1% Gel and purified product
  

Dry Lab

  • Designed primers for LsR promoter
  • Meeting with Dr. Sarkar

June 12th

  

Wet Lab

  • Digested mCherry PCR product with BamHI and NotI
  • Column purified mCherry and ligated into NAS152 backbone
  • Transformed NAS152-mCherry into DH5alpha
  • Poured 25 LB-Kan plates
  

Dry Lab

  • Research more information about bacterial drug delivery system
  • More research into biofilm project

June 14th

  

Wet Lab

  • Fill in later....
  

Dry Lab

  • Met with Dr. Goulian, obtained pDawn and pDusk
  • Identified inaK as a surface display gene we can use

Installation

First, unzip the "vallenato.zip" file and place the "vallenato" folder in the same directory as the html file(s) that will be using the script. If placed in a different folder, you will need to update the paths below.

In the <head> section of your html you will need to link to jQuery, the Vallenato script and stylesheet. You can use the following code:

<script src="http://code.jquery.com/jquery-latest.js" type="text/javascript"></script>

<script src="vallenato/vallenato.js" type="text/javascript"></script>

<link rel="stylesheet" href="vallenato/vallenato.css" type="text/css" media="screen">

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