Team:Penn/Notebook

From 2012.igem.org

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<p><b>June 7th</b></p>
<p><b>June 7th</b></p>
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<li>Meeting with Dr. Sarkar</li>
<li>Meeting with Dr. Sarkar</li>
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<p><b>June 12th</b></p>
<p><b>June 12th</b></p>
                             &nbsp;&nbsp;<p>Wet Lab</p>
                             &nbsp;&nbsp;<p>Wet Lab</p>
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<li>Research more information about bacterial drug delivery system</li>
<li>Research more information about bacterial drug delivery system</li>
<li>More research into biofilm project</li>
<li>More research into biofilm project</li>
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<p><b>June 14th</b></p>
<p><b>June 14th</b></p>
&nbsp;&nbsp;<p>Wet Lab</p>
&nbsp;&nbsp;<p>Wet Lab</p>
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<li>Designed primers for one of Peter's components (forgot which)</li>
<li>Designed primers for one of Peter's components (forgot which)</li>
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<p>June 20</p>
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&nbsp;&nbsp;<p>Wet Lab</p>
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<li>Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan</li>
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<li>PCR purified fragments (Peter), then ran gel?</li>
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&nbsp;&nbsp;<p>Dry Lab</p>
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<ul>
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<li>Researched DARPin binding domains and linkers</li>
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<li>Finalized some biobrick orders</li>
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<li>Finalized synthesis order (minus linker)</li>
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Revision as of 09:44, 15 September 2012

Penn 2012 iGEM Wiki

Drug Delivery Notebook

Expand All | Collapse All

  • June 2012

    • Week 1

      June 6th

      • Set up some lab equipment
      • Autoclaved for a while
      • Organized biobrick stuff
      • Called Vinoo about DNA planning

      June 7th

      • Transformed Cph8, pLsr, and LuxS
      • Placed order with Vinoo
      • Developed idea using PGY/PCN system to activate a gene

    • Week 2

      June 11th

        

      Wet Lab

      • PCR'd mCherry from NAS157
      • Ran 1% Gel and purified product
        

      Dry Lab

      • Designed primers for LsR promoter
      • Meeting with Dr. Sarkar

      June 12th

        

      Wet Lab

      • Digested mCherry PCR product with BamHI and NotI
      • Column purified mCherry and ligated into NAS152 backbone
      • Transformed NAS152-mCherry into DH5alpha
      • Poured 25 LB-Kan plates
        

      Dry Lab

      • Research more information about bacterial drug delivery system
      • More research into biofilm project

      June 14th

        

      Wet Lab

      • Fill in later....
        

      Dry Lab

      • Met with Dr. Goulian, obtained pDawn and pDusk
      • Identified inaK as a surface display gene we can use

    • Week 3

      June 18th

        

      Wet Lab

      • Miniprep pDawn and pDusk
      • Test cut pDawn and pDusk with XmaI, analytical gel was correct
      • Prep cut pDawn and pDusk with BamHI and NotI, gel purified
        

      Dry Lab

      • Ordered and picked up PCR purification kit from cell center
      • Additional orders through cell center
      • Designed primers for one of Peter's components (forgot which)

      June 20

        

      Wet Lab

      • Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
      • PCR purified fragments (Peter), then ran gel?
        

      Dry Lab

      • Researched DARPin binding domains and linkers
      • Finalized some biobrick orders
      • Finalized synthesis order (minus linker)

    • Week 4

      Morbi felis libero, porta non, sagittis ac, consectetur in, sem.
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  • July 2012

    • Week 5

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    • Week 6

      Vestibulum blandit mauris elementum mauris.

    • Week 7

      Morbi felis libero, porta non, sagittis ac, consectetur in, sem.

    • Week 8

      Morbi felis libero, porta non, sagittis ac, consectetur in, sem.

  • August 2012

    • Week 9

      Donec elementum lobortis lorem. Sed aliquet lacus vitae nibh. Sed ullamcorper pharetra augue. Lorem ipsum dolor sit amet, consectetur adipiscing elit.

    • Week 10

      Vestibulum blandit mauris elementum mauris.

    • Week 11

      Morbi felis libero, porta non, sagittis ac, consectetur in, sem.

    • Week 12

      Morbi felis libero, porta non, sagittis ac, consectetur in, sem.

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