Team:WashU/Week12
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<h1>Saffron in a Kan</h1> | <h1>Saffron in a Kan</h1> | ||
We also PCR'd the Z construct in the submission plasmid, Psb1c3. We then digested the results with both EcoRI and PstI, as shown below, and saw that the PCR had indeed succeded.<br> | We also PCR'd the Z construct in the submission plasmid, Psb1c3. We then digested the results with both EcoRI and PstI, as shown below, and saw that the PCR had indeed succeded.<br> | ||
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- | Finally, we ran an SDS-PAGE gel of our Z construct with a histidine tag | + | Finally, we ran an SDS-PAGE gel of our Z construct with a histidine tag in order to see the proteins produced using an extraction protocol from Sigma found here [INSERT PROTOCOL]. |
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- | <table style="width:auto;"><tr><td><a href=" | + | <table style="width:auto;"><tr><td><a href="http://picasaweb.google.com/lh/photo/2iExq1RPSgh9PGJVACqNsT5bkQKfZwcjUCwEKVIJGfc?feat=embedwebsite"><img src="http://lh4.googleusercontent.com/-5UrHwTMRPiM/UCvyrIlsayI/AAAAAAAAAhc/CKYZHdMvoFo/s640/P1060127.JPG" height="428" width="640" /></a></td></tr><tr><td style="font-family:arial,sans-serif; font-size:11px; text-align:right">From <a href="http://picasaweb.google.com/100308169786596215568/August152012?authuser=0&authkey=Gv1sRgCK2xxviFoP6ySA&feat=embedwebsite">August 15, 2012</a></td></tr></table> |
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Since <i>Synechocystis</i> is a photoautotroph, it produces chlorophyll, resulting in the green color. The cyanobacterium also produces beta carotene, the precursor to zeaxanthin, which is the precursor to the crocin we are trying to produce. The peaks for beta carotene and zeaxanthin overlap, so resolving the difference may be difficult. | Since <i>Synechocystis</i> is a photoautotroph, it produces chlorophyll, resulting in the green color. The cyanobacterium also produces beta carotene, the precursor to zeaxanthin, which is the precursor to the crocin we are trying to produce. The peaks for beta carotene and zeaxanthin overlap, so resolving the difference may be difficult. | ||
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Trypsin digestion of ZCD, UGTCS2, Z-construct, and a control<br> | Trypsin digestion of ZCD, UGTCS2, Z-construct, and a control<br> | ||
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- | + | We performed a digest of Z-TOPO and U-TOPO from yesterday, followed by ligation of Z + TOPO, U + TOPO, and our main construct CS42S for submission. | |
- | + | We then transformed our cells with ligation results and plated them. | |
- | + | Finally, we cleaned up our lab and moved items into storage. :( | |
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+ | On our last day, we spent time working on the dry lab stuff and getting our DNA ready for submission and sequencing. | ||
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[https://2012.igem.org/Team:WashU/WeeklyLog Back to Weekly Log] | [https://2012.igem.org/Team:WashU/WeeklyLog Back to Weekly Log] |
Latest revision as of 02:26, 15 September 2012