Team:Tokyo Tech/Projects/Las→Lac assay, Lux→Lac assay/index.htm

From 2012.igem.org

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1.Prepare overnight culture at 37 degrees for 12hours. (2 tubes for each sample)
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2.Take 30 μl of the overnight culture of reporter cell into LB + antibiotics (Amp + Kan). (→fresh culture)
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3.Incubate the flesh culture of reporter cell] until the observed O.D. reaches around 0.60. and gather the supernatant of culture of inducer cell.
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4.Each sample of reporter cell was divided into 2. Prepare and add AHL mixture to one, cell-synthesized AHL to another one, and DMSO mixture to the other.
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5.Induction of reporter cell for 3 hours.
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6.Fluorometer (FLA5200) and flow cytometry measurements for GFP expression of reporter cell
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Latest revision as of 22:25, 14 September 2012

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Las→Lac assay, Lux→Lac assay

Abstract

We succeeded in constructing and characterizing a NEW Biobrick device (name). This device is a LasR generator We found that the device is (explain)

Introduction

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Rusult

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Conclusion

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Material&Method

Construction

Samples

Strain

Protocol

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