Team:Carnegie Mellon

From 2012.igem.org

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<a name="Secondary_Objective:_Humanistic_Practice"></a><h2> <span class="mw-headline"> Secondary Objective: Humanistic Practice</span></h2>
<a name="Secondary_Objective:_Humanistic_Practice"></a><h2> <span class="mw-headline"> Secondary Objective: Humanistic Practice</span></h2>
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<p> <a href="https://2012.igem.org/Team:Carnegie_Mellon" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon" rel="nofollow">our 2012 team</a> in engineering <i>Escherichia coli</i> to <b>be awesome</b>. Here is a link to awesomeness (<a href="http://www.who.int/nutrition/topics/vad/en/" class="external text" title="http://www.who.int/nutrition/topics/vad/en/" rel="nofollow">WHO</a>).
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<p> <a href="https://2012.igem.org/Team:Carnegie_Mellon" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon" rel="nofollow">FAQ/Terminology</a> in engineering <i>Escherichia coli</i> to <b>monitor these variables via fluorescence</b>. Find out more about Carnegie Mellon: (<a href="http://www.cmu.edu" class="external text" title="http://www.cmu.edu" rel="nofollow">CMU Home Page</a>).
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<a name="Further_Considerations"></a><h2> <span class="mw-headline"> Further Considerations </span></h2>
<a name="Further_Considerations"></a><h2> <span class="mw-headline"> Further Considerations </span></h2>
<p>In the pursuit of our project, as well as the biological aspects, we:
<p>In the pursuit of our project, as well as the biological aspects, we:
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<ul><li> considered aspects of <a href="https://2012.igem.org/Team:Carnegie_Mellon/Modelling" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Modelling" rel="nofollow">scale-up</a>, including the <a href="https://2012.igem.org/Team:Carnegie_Mellon" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/ELSI" rel="nofollow">ethical, legal and social implications</a> of our potential final product, MicroMaize,
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<ul><li> considered aspects of <a href="https://2012.igem.org/Team:Carnegie_Mellon/Modelling" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Modelling" rel="nofollow">scale-up</a>, including the <a href="https://2012.igem.org/Team:Carnegie_Mellon" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon" rel="nofollow">ethical, legal and social implications</a> of our BioBrick,
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</li><li> programmed <a href="https://2012.igem.org/Team:Carnegie_Mellon/Software" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Software" rel="nofollow">a new piece of software</a> for use in metabolic modelling,
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</li><li> programmed <a href="https://2012.igem.org/Team:Carnegie_Mellon/Software" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Software" rel="nofollow">a new piece of software</a> for modeling our BioBrick to students,
</li><li> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" rel="nofollow">developed and tested techniques for measuring translational efficiency and transcriptional strength,
</li><li> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" rel="nofollow">developed and tested techniques for measuring translational efficiency and transcriptional strength,
</li><li> participated in human practices demonstration xxx.
</li><li> participated in human practices demonstration xxx.

Revision as of 11:40, 11 June 2012

Team:Carnegie_Mellon - 20012.igem.org

Team:Carnegie_Mellon

From 2012.igem.org

Contents

Introduction: Motivation

  • We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.
  • We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.
  • We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.


Abstract/Introduction

Motivation question

Humanistic implications go here

Primary Objective: A Useful BioBrick for Synthetic Biologists

A false-colour SEM of MicroMaize cells.
A false-colour SEM of MicroMaize cells.

We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications, for a variety of purposes such as the following:

  1. example application
  2. example application
  3. example application

Our proposed BioBrick is novel, and potentially very useful in practice.

Secondary Objective: Humanistic Practice

FAQ/Terminology in engineering Escherichia coli to monitor these variables via fluorescence. Find out more about Carnegie Mellon: (CMU Home Page).

Further Considerations

In the pursuit of our project, as well as the biological aspects, we:

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