Team:Goettingen/week7-3

From 2012.igem.org

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<li>Experiment: <br>We selected 8 different promoters for our chemoreceptor TAR. We chose the following parts for this purpose: <br>  
<li>Experiment: <br>We selected 8 different promoters for our chemoreceptor TAR. We chose the following parts for this purpose: <br>  
J23100 (18C) <br> J23104 (18K) <br> J23105 (18M) <br> J23106 (18O) <br> J23109 (2G) <br> J23112 (20E) <br> J23113 (20G) <br> J23114 (20I) <br> all of these in vector J61002 (for details click <a href="http://partsregistry.org/Main_Page">here</a>). These constructs were, prior to this experiment, transformed into <i>E. coli</i>, prepped and stored at -20 °C. <br>
J23100 (18C) <br> J23104 (18K) <br> J23105 (18M) <br> J23106 (18O) <br> J23109 (2G) <br> J23112 (20E) <br> J23113 (20G) <br> J23114 (20I) <br> all of these in vector J61002 (for details click <a href="http://partsregistry.org/Main_Page">here</a>). These constructs were, prior to this experiment, transformed into <i>E. coli</i>, prepped and stored at -20 °C. <br>
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The vector J61002 was digested with <i>Spe</i>I and <i>Pst</i>I, the insert TAR_QC was digested with <i>Xba</i>I and <i>Pst</i>I according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>.
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The vector J61002 was digested with <i>Spe</i>I and <i>Pst</i>I, the insert TAR_QC was digested with <i>Xba</i>I and <i>Pst</i>I and subsequently ligated downstream of our 8 promoters according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. We transformed <i>E. coli</i> &Delta; <i>tar</i> with the generated constructs.
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<tr bordercolor="black" valign="top">
<tr bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
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<h2><b>V06_12 </b></h2><br>
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<h2><b>V06_13 </b></h2><br>
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<b>bla</b><br>
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<b>Preparation of J61002_promoter_TAR_QC</b><br>
<ul>
<ul>
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<li>Experiment: <br>bla
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<li>Experiment: <br>All 8 promoter constructs + TAR_QC were prepped using PeqGOLD Plasmid Miniprep kit (PeqLab) following the user manual. We performed a test digestion using <i>Xba</i>I and <i>Pst</i>I to verify that the insert TAR_QC was successfully integrated into the plasmid.
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Revision as of 10:44, 14 September 2012

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