Team:Goettingen/week7-2

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Revision as of 10:42, 14 September 2012

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V06_11_1 Miniprep of the new flhDC-promoter constructs

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_11_2 Chemical retransformartion of the new flhDC-promoter constructs into E. coli (DH10B)

Experiment:
For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:
20G - #1
20G - #2
2G - #1
2G - #2
2G - #3
20I - #2
20I - #3
18O - #2
18O - #3
18K - #1
18K - #2
18K - #3
Observations & Results:
The retransformation was successful since all plates showed numeours colonoes except the negative control.

Preparation of over night cultures

V06_13_1 Miniprep of the new flhDC-promoter constructs

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
V06_013_2 Chemical transformartion of pBAD-sfGFP into E. coli (DH10B)
- Experiment:
  For the chemical transformation the standard protocol was followed.
- Observations & Results:
  The transformation was successful since all plates showed numeours colonoes except the negative control.

Preparation of over night cultures

Experiment:
Over night cultures of the pBAD-sfGFP clones were prepared in order to isolate the plasmids.

Miniprep of pBAD-sfGFP

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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-<b>V06_013_2 Chemical transformartion of pBAD-glGFP into E. coli (DH10B)</b><br>
+<b>V06_013_2 Chemical transformartion of pBAD-<i>sfGFP</i> into E. coli (DH10B)</b><br>
 <ul>
 <li>Experiment:  <br>
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 </table>
+<br>
+<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
+<tr bordercolor="black" valign="top">
+<td width="900" bordercolor="black" valign="top">
 <h2><b>V06_14 </b></h2><br>
-<b>Preparation of over night cultures</i></b><br>
+<b> Preparation of over night cultures</i></i></b><br>
+<ul>
+<li>Experiment: <br>
+Over night cultures of the pBAD-<i>sfGFP</i> clones were prepared in order to isolate the plasmids. <br>
+<br></td></tr>
+</table>
+<br>
+<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
+<tr bordercolor="black" valign="top">
+<td width="900" bordercolor="black" valign="top">
+<h2><b>V06_15 </b></h2><br>
+<b> Miniprep of pBAD-<i>sfGFP</i></i></i></b><br>
 <ul>
 <li>Experiment: <br>
-Over night cultures of pBAD-glGFP were prepared in order to isolate the plasmids. <br>
+Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. <br>
 <br></td></tr>
 </table>