Team:Goettingen/week7-2

V06_11

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

• Experiment:
  For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:
  20G - #1
  20G - #2
  2G - #1
  2G - #2
  2G - #3
  20I - #2
  20I - #3
  18O - #2
  18O - #3
  18K - #1
  18K - #2
  18K - #3
  
• Observations & Results:
  The retransformation was successful since all plates showed numeours colonoes except the negative control.

V06_12

• Experiment:
  In order to gain further plasmid material over night cultures were prepared for subsequent plasmid isolation.
  

V06_07

• Experiment:
  Over night cultures were preaped of three colonies of each constructs in order to isolate the plasmids.
  

V06_08

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

• Experiment:
  In order to prove correct insertion of flhDC a test digestion was performed using SpeI and XbaI according to the protocol.
  
• Observations & Results:
  For each construct two to three clones hosting the correctly inserted gene in the plasmid could be obtained. Those colonies still containing the rfp gene and were thus rejected.