"
Team:Potsdam Bioware
From 2012.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
<html> | <html> | ||
| + | <div class="box_round gradient_grey"> | ||
<h2>Direct Antibody Identification, Maturation and Production in CHO-Cells</h2> | <h2>Direct Antibody Identification, Maturation and Production in CHO-Cells</h2> | ||
| - | + | </div> | |
| + | <br> | ||
<div class="box_round gradient_grey"> | <div class="box_round gradient_grey"> | ||
<h3>Project Description</h3> | <h3>Project Description</h3> | ||
Revision as of 14:02, 13 September 2012
Direct Antibody Identification, Maturation and Production in CHO-Cells
Project Description
Antibodies are versatile molecules for research and therapy and they are currently revolutionizing the market of biopharmaceuticals. To date, antibodies are generated in a laborious and time consuming manner. Either animal immunization followed by hybridoma technology or phage display is used to identify desired genes, which then need to be transferred to a production cell line such as CHO. We designed a streamlined workflow that incorporates all steps of antibody generation in CHO cells. Our plan is to stably transfect an antibody-fragment library in CHO cells. Antibody maturation is mimicked by further diversifying this library using the enzyme Activation-Induced cytidine Deaminase (AID), which is known to induce somatic hypermutation. Next, we plan to test and deploy a versatile and continuous viral selection system for clones producing the desired antibodies. Finally, we will try to employ a genetic switch to go from surface expression to soluble production of antibodies.
Team
