Team:EPF-Lausanne/Protocol/PCR

From 2012.igem.org

(Difference between revisions)
Line 9: Line 9:
1X Mastermix 20μl reaction
1X Mastermix 20μl reaction
-
# Water 20μl - x
+
20μl - x
-
# HF-Buffer (5x) 4μl
+
# DMSO (optional) 0.6μl
# DMSO (optional) 0.6μl
#dNTPs 0.4μl
#dNTPs 0.4μl
Line 19: Line 19:
#Phusion HF polymerase 0.2μl
#Phusion HF polymerase 0.2μl
 +
 +
{| class="wikitable"
 +
|-
 +
! Reagent
 +
! [μl]
 +
|-
 +
|Water
 +
| 20-x
 +
|-
 +
|HF-Buffer (5x)
 +
|4
 +
|-
 +
|DMSO (optional)
 +
|0.6
 +
|-
 +
|dNTPs
 +
|0.4
 +
|-
 +
|Forward primer (50μM)
 +
|0.2
 +
|-
 +
|Reverse primer (50μM)
 +
|0.2
 +
|-
 +
|Template (10ng/μl)
 +
|0.5
 +
|-
 +
|Phusion HF polymerase
 +
|0.2
 +
|}
{{:Team:EPF-Lausanne/Template/ProtocolFooter}}
{{:Team:EPF-Lausanne/Template/ProtocolFooter}}
<noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude>
<noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude>

Revision as of 09:57, 13 September 2012

Protocol: PCR

1X Mastermix 20μl reaction

20μl - x

  1. DMSO (optional) 0.6μl
  2. dNTPs 0.4μl
  3. Primers (50μM)
    1. Forward 0.2μl
    2. Reverse 0.2 μl
  4. Template (10ng/μl) 0.5μl
  5. Phusion HF polymerase 0.2μl


Reagent [μl]
Water 20-x
HF-Buffer (5x) 4
DMSO (optional) 0.6
dNTPs 0.4
Forward primer (50μM) 0.2
Reverse primer (50μM) 0.2
Template (10ng/μl) 0.5
Phusion HF polymerase 0.2

Wiki Links

Important pages

Pages

Retrieved from "http://2012.igem.org/Team:EPF-Lausanne/Protocol/PCR"