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Team:KAIT Japan/Notebook
From 2012.igem.org
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__NOTOC__ | __NOTOC__ | ||
| - | =Creating parts of Tar methylation region= | + | ='''Notebook'''= |
| - | ==Date:8/9== | + | ==Creating parts of Tar methylation region== |
| + | ===Date:8/9=== | ||
Colony PCR | Colony PCR | ||
::'''→Reflection:We Took E.coli too many.You should take less E.coli.'''<br> | ::'''→Reflection:We Took E.coli too many.You should take less E.coli.'''<br> | ||
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---- | ---- | ||
| - | ==Date:8/11== | + | ===Date:8/11=== |
The purified DNA | The purified DNA | ||
::'''→Reflection:Band was less.''' | ::'''→Reflection:Band was less.''' | ||
| Line 67: | Line 68: | ||
---- | ---- | ||
| - | ==Date:8/13== | + | ===Date:8/13=== |
Confirmed of electrophoresis by PCR product and Ligation of the TA vector | Confirmed of electrophoresis by PCR product and Ligation of the TA vector | ||
---- | ---- | ||
| - | ==Date:8/14== | + | ===Date:8/14=== |
Transformation | Transformation | ||
---- | ---- | ||
| - | ==Date:8/25== | + | ===Date:8/25=== |
Miniprep | Miniprep | ||
| - | =Creating parts of azurin= | + | ==Creating parts of azurin== |
| - | ==Date:8/16== | + | ===Date:8/16=== |
Colony PCR | Colony PCR | ||
Ligation | Ligation | ||
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---- | ---- | ||
| - | ==Date:8/18== | + | ===Date:8/18=== |
Colony PCR | Colony PCR | ||
---- | ---- | ||
| - | ==Date:8/20== | + | ===Date:8/20=== |
DNA extraction and purification of ''P.aeruginosa'' | DNA extraction and purification of ''P.aeruginosa'' | ||
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Revision as of 03:59, 13 September 2012
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NotebookCreating parts of Tar methylation regionDate:8/9Colony PCR
Date:8/11The purified DNA
PCR Product Date:8/13Confirmed of electrophoresis by PCR product and Ligation of the TA vector Date:8/14Transformation Date:8/25Miniprep Creating parts of azurinDate:8/16Colony PCR Ligation Date:8/18Colony PCR Date:8/20DNA extraction and purification of P.aeruginosa |
