Team:Wisconsin-Madison

From 2012.igem.org

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      <p class = "classtheoverview"> The goal and introduction of iGEM @ Wisconsin-Madison 2012.
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        The goal and introduction of iGEM @ Wisconsin-Madison 2012.
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        The goal and introduction of iGEM @ Wisconsin-Madison 2012.
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            iGEM team University Wisconsin-Madison 2012 has ? members, ? projects, and ...  
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        The goal and introduction of iGEM @ Wisconsin-Madison 2012. </p>
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        <strong>Ryan</strong><br>
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Ryan is a senior in the chemical engineering department at UW-Madison. Originally he was interested in industrial research, but synthetic biology soon stole his heart. When not in the lab, Ryan can be spotted in many places on campus. He spends late nights in the concrete canoe lab, mixing and sanding concrete repeatedly. He also is a tour guide and tutors for organic chemistry. Ryan intends to go to grad school after his last year at Madison, hopefully to pursue his passion for synthetic biology.
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        <strong>Michael</strong><br>
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            Michael is a sophomore in the biochemistry department with both math and physics minors. After working in labs at the Mayo Clinic during high school, and receiving an award at the International Science and Engineering Fair for his immunology research done there, Michael jumped straight into research upon arriving at the UW. Michael has for many years had a passion for synthetic biology and the potential in holds. In his spare time Michael can be found fencing, larping, role-playing, reading, or otherwise being a nerd. Michael plans to go to grad school, pursuing a PhD with every plan of returning to synthetic biology in his future.
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        <strong>Chris</strong><br>
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            Chris is a senior in Microbiology, minoring in Business Entrepreneurship, and is interested in biofuels, biorefining, and the future of the energy economy. He has done research in materials science, pharmaceutical discovery, and unconventional yeast metabolism. When not pondering the fate of humanity, Chris enjoys swimming, water polo, and sailing big boats.
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      <p class="classtheinlinecontent">Limonene is a colorless, liquid hydrocarbon that smells like lemon. It is found in the oils of citrus fruits and is primarily used as a cleaning agent, solvent, and flavorful food additive. Limonene also possesses chemical properties that make it an ideal jet fuel due to it's low freezing point and high combustibility. E. coli already has part of the metabolic pathway required to produce limonene, however we will be inserting genes from S. cerevisiae to create the mevalonate pathway in the cell. We will also codon optimize the Limonene Synthase (LimS1) gene which is the final step in converting GPP to limonene. Gas chromatography/mass spectrometry will be used to determine how much limonene the E. coli is producing. </p>
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        <strong>Charles</strong><br>
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        Charles Johnson is a Junior in the department of Chemical and Biological Engineering at UW-Madison. He is interested in studying the many different ways synthetic biology can be used to help the world, from medical applications to biofuels. When Charlie is not in the lab he wants fallow in his family's footsteps by helping those less fortunate than him, his older brother and grandmother dedicated their lives to teaching in the inner city of Chicago, while his parents have been representing people who could not afford lawyers for 30 plus years, and his younger brother works in a group home helping abused and neglected children get a safe place to live and learn.
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        <strong>Eric</strong><br>
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        Eric was on the 2011 UW-Madison iGEM team, and returned this year as a mentor. He is interested in developing novel, robust genetic systems and circuits. He spent much of his undergraduate career developing systems for engineering cyanobacteria, and is leaving us in the fall to begin the PhD program at UC Davis in the Microbiology Graduate Group. When he's not busy tweeting as @LabBadger or finding new, dreadful music, he's usually out riding his rusty bike through alleys full of broken glass.
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        <strong>Menuka</strong><br>
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        Menuka Samaranayake is a senior at the University of Wisconsin-Madison is double-majoring in Microbiology and Political Science. This is Menuka's first foray into the huge, exciting world of conducting research, and he feels that this opportunity of competing in iGEM has been an invaluable experience. Menuka has no idea what his career goals are, but would at some point work in the government.  In his spare time, he likes to chill with cats
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        <strong>Sophia</strong><br>
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        <strong>Klaus</strong><br>
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        <strong>Yishen</strong><br>
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        Yishen is a sophomore in Biomedical Engineering department of University of Wisconsin Madison. He decided to study biology:  genetic engineering in specific around middle school. After finishing up his high school education in China, he traveled to the U.S. and became a student of University of Wisconsin. He wishes that in the future, he could become a better scientist at synthetic biology and genetic engineer so that he could convey many of his ideas much more effectively and efficiently. In his spare time, Yishen would prefer spending his time exercising Chinese kung fu in the hope of better physical condition and a more peaceful state of mind.
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        <strong>Danielle</strong><br>
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        <strong>Chelsea</strong><br>
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        <strong>Matt</strong><br>
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        <strong>Matt Copeland, PhD</strong><br>
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        <strong>PI: Brian Pfleger, PhD</strong><br>
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SURFACE DISPLAY AND FLOCCULATION
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Engineering an easy cut and paste method to display proteins
 
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Many previous iGEM teams have had success displaying a number of different enzymes and proteins on the surface of a cell. However, many of the methods used were specific to a lab at their respective universities. We wanted to create a common “display vector” that all teams can use to display any part they would like. This vector also allows for the use of standard digestion and ligation protocols, and not newer cloning methods like Gibson or PIPE. We also wanted to characterize a surface displayed Beta-Glucosidase part which would allow the degradation of cellobiose, which has large implications for the alternative fuel industry.
 
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Revision as of 03:57, 13 September 2012


iGEM team University Wisconsin-Madison 2012 has ? members, ? projects, and ...

Ryan

Ryan is a senior in the chemical engineering department at UW-Madison. Originally he was interested in industrial research, but synthetic biology soon stole his heart. When not in the lab, Ryan can be spotted in many places on campus. He spends late nights in the concrete canoe lab, mixing and sanding concrete repeatedly. He also is a tour guide and tutors for organic chemistry. Ryan intends to go to grad school after his last year at Madison, hopefully to pursue his passion for synthetic biology.

Michael

Michael is a sophomore in the biochemistry department with both math and physics minors. After working in labs at the Mayo Clinic during high school, and receiving an award at the International Science and Engineering Fair for his immunology research done there, Michael jumped straight into research upon arriving at the UW. Michael has for many years had a passion for synthetic biology and the potential in holds. In his spare time Michael can be found fencing, larping, role-playing, reading, or otherwise being a nerd. Michael plans to go to grad school, pursuing a PhD with every plan of returning to synthetic biology in his future.

Chris

Chris is a senior in Microbiology, minoring in Business Entrepreneurship, and is interested in biofuels, biorefining, and the future of the energy economy. He has done research in materials science, pharmaceutical discovery, and unconventional yeast metabolism. When not pondering the fate of humanity, Chris enjoys swimming, water polo, and sailing big boats.

Charles

Charles Johnson is a Junior in the department of Chemical and Biological Engineering at UW-Madison. He is interested in studying the many different ways synthetic biology can be used to help the world, from medical applications to biofuels. When Charlie is not in the lab he wants fallow in his family's footsteps by helping those less fortunate than him, his older brother and grandmother dedicated their lives to teaching in the inner city of Chicago, while his parents have been representing people who could not afford lawyers for 30 plus years, and his younger brother works in a group home helping abused and neglected children get a safe place to live and learn.

Eric

Eric was on the 2011 UW-Madison iGEM team, and returned this year as a mentor. He is interested in developing novel, robust genetic systems and circuits. He spent much of his undergraduate career developing systems for engineering cyanobacteria, and is leaving us in the fall to begin the PhD program at UC Davis in the Microbiology Graduate Group. When he's not busy tweeting as @LabBadger or finding new, dreadful music, he's usually out riding his rusty bike through alleys full of broken glass.

Menuka

Menuka Samaranayake is a senior at the University of Wisconsin-Madison is double-majoring in Microbiology and Political Science. This is Menuka's first foray into the huge, exciting world of conducting research, and he feels that this opportunity of competing in iGEM has been an invaluable experience. Menuka has no idea what his career goals are, but would at some point work in the government. In his spare time, he likes to chill with cats

Sophia

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Klaus

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Yishen

Yishen is a sophomore in Biomedical Engineering department of University of Wisconsin Madison. He decided to study biology: genetic engineering in specific around middle school. After finishing up his high school education in China, he traveled to the U.S. and became a student of University of Wisconsin. He wishes that in the future, he could become a better scientist at synthetic biology and genetic engineer so that he could convey many of his ideas much more effectively and efficiently. In his spare time, Yishen would prefer spending his time exercising Chinese kung fu in the hope of better physical condition and a more peaceful state of mind.

Danielle

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Chelsea

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Matt

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Matt Copeland, PhD

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PI: Brian Pfleger, PhD

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