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Team:Chalmers-Gothenburg/Results
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An initial test was performed in order to test the survival of yeast cells in urine, which is an important property of our biosensor if it should function as a pregnancy test. Pre-cultures of a normal lab yeast strain were prepared in 5ml YPD and let grown O/N at 30 °C. The day after, the cells were centrifuged, resuspended in 40 ml YPD and 40 ml filter sterilized urine respectively and grown in shake flasks at 37°C. OD600 measurements were taken at different points of time during the day, and the values can be seen in Figure 1. The cells in urine did not grow; the OD remained the same during the whole day, while the cells in YPD grew very well. | An initial test was performed in order to test the survival of yeast cells in urine, which is an important property of our biosensor if it should function as a pregnancy test. Pre-cultures of a normal lab yeast strain were prepared in 5ml YPD and let grown O/N at 30 °C. The day after, the cells were centrifuged, resuspended in 40 ml YPD and 40 ml filter sterilized urine respectively and grown in shake flasks at 37°C. OD600 measurements were taken at different points of time during the day, and the values can be seen in Figure 1. The cells in urine did not grow; the OD remained the same during the whole day, while the cells in YPD grew very well. | ||
| - | [File:Growth_curve_urine.jpg|frame|Figure 1: Growth curve for cells in Urine and YPD medium. One can see that the OD remains the same in the urine i.e. that the cells are not growing.] | + | [[File:Growth_curve_urine.jpg|frame|Figure 1: Growth curve for cells in Urine and YPD medium. One can see that the OD remains the same in the urine i.e. that the cells are not growing.]] |
=='''Expression of human LH/CG receptor'''== | =='''Expression of human LH/CG receptor'''== | ||
Revision as of 18:44, 12 September 2012
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Contents |
Results Summary
Survival of yeast in urine
An initial test was performed in order to test the survival of yeast cells in urine, which is an important property of our biosensor if it should function as a pregnancy test. Pre-cultures of a normal lab yeast strain were prepared in 5ml YPD and let grown O/N at 30 °C. The day after, the cells were centrifuged, resuspended in 40 ml YPD and 40 ml filter sterilized urine respectively and grown in shake flasks at 37°C. OD600 measurements were taken at different points of time during the day, and the values can be seen in Figure 1. The cells in urine did not grow; the OD remained the same during the whole day, while the cells in YPD grew very well.
Figure 1: Growth curve for cells in Urine and YPD medium. One can see that the OD remains the same in the urine i.e. that the cells are not growing.
Expression of human LH/CG receptor
Introduction of indigo synthesizing genes
Deletion of CWP2 gene
