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Team:LMU-Munich/Attributions
From 2012.igem.org
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| - gDNA from ''B. subtilis'' W168 | | - gDNA from ''B. subtilis'' W168 | ||
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| - | | - Several plasmids (pDG148, pDG1716, pAC6, pDG1662, [plasmids as templates for resistance cassettes]) | + | | - Several plasmids (pDG148, pDG1716, pAC6, pDG1662, pAH328, pXT, pCSlux103, [plasmids as templates for resistance cassettes]) |
|- | |- | ||
| - Several primers | | - Several primers | ||
|- | |- | ||
| + | | - all main protocols how to work with ''B. subtilis'' | ||
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| - We used plasmids from the [http://www.bgsc.org/ Bacillus Genetic Stock Center] as template for our [[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|new backbone vectors]]. | | - We used plasmids from the [http://www.bgsc.org/ Bacillus Genetic Stock Center] as template for our [[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|new backbone vectors]]. | ||
| + | |- | ||
| + | | - We were allowed to use the microscopes from AG Bramkamp, after a thorough introduction. The pictures were taken by members od our team. | ||
| + | |- | ||
| + | | - Some microscopy protocols from AG Bramkamp | ||
|} | |} | ||
Revision as of 20:20, 11 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Attributions
Here we attribute work done by others and ourselves. We distinguish work done by the team from work done by others, including the Mascher Work Group (our host lab), LMU lab groups, advisors, instructors, graduate students, and postgraduate masters students.
| Materials we have received from the Mascher Work Group: |
| - Bacillus subtilis WT strain W168 |
| - gDNA from B. subtilis W168 |
| - Several plasmids (pDG148, pDG1716, pAC6, pDG1662, pAH328, pXT, pCSlux103, [plasmids as templates for resistance cassettes]) |
| - Several primers |
| - all main protocols how to work with B. subtilis |
| Work we have received from others: |
| - Introduction to fluorescence microscopy by the lab of [http://www.bacteriology.bio.lmu.de/ Marc Brahmkamp] |
| - [http://jb.asm.org/content/193/16/4075 Imamura et al.]: We received plasmids from his work with the spore crust proteins that we can use as a positive control. The plasmids include cotZ-gfp and cgeA-rfp fusion proteins and a gfp, that is produced inside the spore. |
| - Mathias Plichta helped us with the technical implementation of the website |
| - We used the experience and some materials from last year's LMU-Munich iGEM team for our highschool practical course. |
| - Sequencing from [http://www.gi.bio.lmu.de/sequencing Dr. Andreas Brachmann] |
| - We used plasmids from the [http://www.bgsc.org/ Bacillus Genetic Stock Center] as template for our new backbone vectors. |
| - We were allowed to use the microscopes from AG Bramkamp, after a thorough introduction. The pictures were taken by members od our team. |
| - Some microscopy protocols from AG Bramkamp |
| Work we have done ourselves: |
| - Promoter evaluations: Korinna Kraft |
| - Construction of our new BioBricks |
Project Navigation
| +--+--+--+--+--+--+-- | +--+--+--+--+-- | +--+--+--+--+--+-- | +--+--+--+--+--+-- |
Bacillus BioBrickBOX |
SporeCoat FusionProteins |
Germination STOP |
