Team:LMU-Munich/Lab Notebook/Protocols

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Revision as of 13:56, 11 September 2012

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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Protocols

On this page, we offer our unique protocols to the public.

Because Bacillus subtilis is our choice organism, we have focused on protocols specifically for Bacillus.

1. Media and components for Bacillus subtilis

This protocol gives the recipes for various media and the antibiotic concentrations used for B. subtilis.

2. Transformation of Bacillus subtilis

Protocol for the transformation of B. subtilis.

3. Isolation of chromosomal DNA from Bacillus subtilis for transformation

A quick protocol for how to isolate genomic DNA from B. subtilis to transform it to a different strain. By this means, you can very easily combine different B. subtilis genotypes. Transformation of genomic DNA is much more efficient than plasmids. Isolated DNA with this protocol is not clean!

4. Isolation of chromosomal DNA from Bacillus subtilis (for PCR....)

Isolation of pure genomic DNA from B. subtilis.

5. Long Flanking Homology PCR

PCR protocol for long flanking homology PCR. You might need those PCR products for interrupting genes with resistance cassettes.

6. Clean deletion of genomic fragments in Bacillus subtilis

Protocol for the production of markerless gene deletions. You will need the [http://www.ncbi.nlm.nih.gov/pubmed/15528558 pMAD] plasmid. This method is more time consuming than inserting resistance cassettes but offers better cell perfomance.

7. Spore germination assay

Protocol to count efficiency of sporulation and germination.

8. ß-Galactosidase Assay B. subtilis

Protocol how to measure lacZ gene activity in B. subtilis

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Lab_Notebook/Protocols"

Revision as of 13:54, 11 September 2012 (view source) Jennifer (Talk \| contribs) ← Older edit		Revision as of 13:56, 11 September 2012 (view source) Jennifer (Talk \| contribs) Newer edit →
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	3. [https://static.igem.org/mediawiki/2012/0/00/LMU-Munich_2012_Isolation_of_chromosomal_DNA_from_Bacillus_subtilis_for_transformation.pdf Isolation of chromosomal DNA from ''Bacillus subtilis'' for transformation]		3. [https://static.igem.org/mediawiki/2012/0/00/LMU-Munich_2012_Isolation_of_chromosomal_DNA_from_Bacillus_subtilis_for_transformation.pdf Isolation of chromosomal DNA from ''Bacillus subtilis'' for transformation]

-	A quick protocol how to isolate genomic DNA from ''B.subtilis'' to transform it to a different strain. By this ~~mean~~ you can very easily combine different B. subtilis genotypes. ~~transformation~~ of genomic DNA is much more efficient than plasmids. Isolated DNA with this protocol is not clean!	+	A quick protocol for how to isolate genomic DNA from ''B. subtilis'' to transform it to a different strain. By this means, you can very easily combine different ''B. subtilis'' genotypes. Transformation of genomic DNA is much more efficient than plasmids. Isolated DNA with this protocol is not clean!