Team:Macquarie Australia/Protocols/ResultsofGibsonsofar110912

From 2012.igem.org

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Results from sequencing at the Macquarie facility are as follows:
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==='''Results'''===
* T7 Heme oxygenase (chloramphenicol): This biobrick assembly was successful. The plasmids will be sequenced.
* T7 Heme oxygenase (chloramphenicol): This biobrick assembly was successful. The plasmids will be sequenced.
* Heme oxygenase (Kanamycin): Incubation is to be performed today and plasmid preparation using the Qiagen protocol will be performed. No colonies were present on the T7 minus heme oxygenase on ampicillin.
* Heme oxygenase (Kanamycin): Incubation is to be performed today and plasmid preparation using the Qiagen protocol will be performed. No colonies were present on the T7 minus heme oxygenase on ampicillin.

Revision as of 02:43, 11 September 2012




Results

  • T7 Heme oxygenase (chloramphenicol): This biobrick assembly was successful. The plasmids will be sequenced.
  • Heme oxygenase (Kanamycin): Incubation is to be performed today and plasmid preparation using the Qiagen protocol will be performed. No colonies were present on the T7 minus heme oxygenase on ampicillin.
  • Deinococcus: Plasmid sequencing will be performed and transformation was successful on kanamycin plates. The Deinococcus on the kanamycin was unsuccessful.
  • T7 Agrobacterium: no colonies were present on the chloramphenicol plates.
  • Agrobacterium: no colonies were present on either Kanamycin or Ampicillin plates.