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Team: Macquarie Australia/Protocols/SchoolVisit
From 2012.igem.org
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Team members made up more LB media in order to 'draw' on plates with the fluorescent bacteria for Open Day on Saturday. The following protocol was used as previously done in earlier steps. | Team members made up more LB media in order to 'draw' on plates with the fluorescent bacteria for Open Day on Saturday. The following protocol was used as previously done in earlier steps. | ||
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| + | LB media: (1L total) | ||
| + | Ingredients: Tryptone 10g, Yeast extract 5g, NaCl 10g, MilliQ water to 1000ml. | ||
| + | Methods: Dissolved 10g tryptone, 5g yeast extract and 10g NaCl in 800 mL MilliQ water, making use of a magnetic stirrer. Once dissolved, brought volume up to 1L using MilliQ water. Autoclaved 1000ml of the solution (121°C, 15 min, standard liquid cycle). | ||
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| + | The LB media was used to inoculate the fluorescent bacteria in order to draw on the plates. Below are pictures of the resulting fluorescent bacteria drawings under UV light for Open Day. | ||
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Latest revision as of 01:53, 11 September 2012
Visit to Green Point Christian College
Today four team members attended Green Point Christian College to present a workshop on Synthetic Biology.
Outreach Administration: Open Day on 8th September
Allocated team members were performing final preparations for the Macquarie University Open Day on Saturday 8th September. Below are materials created by team members to use to educate the public.
Making more plates for Open Day 8th September
Team members made up more LB media in order to 'draw' on plates with the fluorescent bacteria for Open Day on Saturday. The following protocol was used as previously done in earlier steps.
LB media: (1L total) Ingredients: Tryptone 10g, Yeast extract 5g, NaCl 10g, MilliQ water to 1000ml. Methods: Dissolved 10g tryptone, 5g yeast extract and 10g NaCl in 800 mL MilliQ water, making use of a magnetic stirrer. Once dissolved, brought volume up to 1L using MilliQ water. Autoclaved 1000ml of the solution (121°C, 15 min, standard liquid cycle).
The LB media was used to inoculate the fluorescent bacteria in order to draw on the plates. Below are pictures of the resulting fluorescent bacteria drawings under UV light for Open Day.
