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Team:MIT/MaterialsAndMethods
From 2012.igem.org
(Difference between revisions)
| Line 77: | Line 77: | ||
<h1> Gate Anneals </h1> | <h1> Gate Anneals </h1> | ||
<u> Room Temperature Annealing </u> | <u> Room Temperature Annealing </u> | ||
| - | <OL> | + | <OL> |
| - | <LI>Mix gate and output in a 1.2:1 ratio in a tube.</LI> | + | <LI>Mix gate and output in a 1.2:1 ratio in a tube.</LI> |
| - | + | <LI>Cover the tube in foil, if you didn't use an opaque/black tube.</LI> | |
| - | + | <LI>Leave the tube on a bench top overnight.</LI> | |
</OL> | </OL> | ||
Revision as of 22:50, 9 September 2012
Gate Anneals
Room Temperature Annealing- Mix gate and output in a 1.2:1 ratio in a tube.
- Cover the tube in foil, if you didn't use an opaque/black tube.
- Leave the tube on a bench top overnight.
