Team:UIUC-Illinois/Notebook/Protocols/Inoculation

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<h1><center>Select Protocol</center></h1>

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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/Bootcamp">Bootcamp Protocols</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/Subculturing">Subculturing Plates</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/Electrocompetence">Making Electrocompetent E.Coli</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/TAE">Making TAE Buffers</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/GelElectrophoresis">Making Electrophoresis Gels</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/GelPurification">Gel Purification</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/Inoculation">Inoculation</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/Miniprep">Miniprep</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/PCR">PCR Protocols</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/Digestions">Digestions</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/Ligations">Ligations</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/Transformation">Transformation of E.Coli</a>
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 <a href="https://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/Storage">Storage of Cells</a>
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<center><h1>Inoculation</h1></center><br/>
What you need:<br/>
- Falcon tubes<br/>
- The appropriate liquid media<br/>
- The appropriate antibiotic<br/>
- An agar plate containing the cells of interest or a frozen stock solution<br/>
- Autoclaved pipet tips<br/><br/>
Procedure: <br/><br/>
1. To an autoclaved glass test tube with lid, add: 2-5 mL liquid media, 2-5 ul antibiotic (dilute to 1/1000)<br/>
2. Using a pipet tip,  gently poke a single colony of plated cells, or scratch cells onto the stick form the frozen freezer stock (taking colonies from a plate typically yields better results)<br/>
3. Stir cells into the liquid media<br/>
4. Label the test tubes and place them in 37˚C warm room overnight to grow (12-16 hrs)<br/><br/>


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