Team:NYU Gallatin/Project/Cloning/Protocols/Transformation

{{:Team:NYU_Gallatin/Templates/Styles}}
<html><body>
<div class="html not-front not-logged-in one-sidebar sidebar-first page-node page-node- page-node-33 node-type-page" >
<div id="top-main-menu" class="navigation">
  <h2 class="element-invisible">Main menu</h2><ul id="top-main-menu-links" class="links clearfix"><li class="menu-218 first"><a href="/Team:NYU_Gallatin/" title="Home of Aseatobacter.">Home</a></li>
<li class="menu-388"><a href="/Team:NYU_Gallatin/Team" title="The brains of the operation.">Team</a></li>
<li class="menu-307"><a href="/Team:NYU_Gallatin/Project" title="Learn more about our project.">Project</a></li>
<li class="menu-308"><a href="/Team:NYU_Gallatin/Parts" title="Our work with the parts registry.">Parts</a></li>
<li class="menu-310"><a href="/Team:NYU_Gallatin/Modeling" title="How we put it all together.">Modeling</a></li>
<li class="menu-311"><a href="/Team:NYU_Gallatin/Notebook" title="Lab notebooks, news, &amp; photos.">Notebook</a></li>
<li class="menu-312"><a href="/Team:NYU_Gallatin/Safety" title="Our commitment to safety.">Safety</a></li>
<li class="menu-313"><a href="/Team:NYU_Gallatin/Attributions" title="Give credit where credit is due.">Attributions</a></li>
<li class="menu-306 last"><a href="https://igem.org/Team.cgi?year=2012&amp;team_name=NYU_Gallatin" title="Official iGEM 2012 profile.">Profile</a></li>
</ul></div> <!-- /#main-menu -->
<div id="page-wrapper"><div id="page">
   
</html>
{{:Team:NYU_Gallatin/Templates/Header_Project}}
<html><nowiki>
    
       <div id="main-wrapper" class="clearfix"><div id="main" class="clearfix"><div id="main-inner">
          <div id="sidebar-first" class="column sidebar"><div class="section">
          <div class="region region-sidebar-first">
    <div id="block-menu-menu-project-menu" class="block block-menu">

    <h2>The Project</h2>
  
  <div class="content">
    <ul class="menu clearfix"><li class="first last expanded"><a href="/Team:NYU_Gallatin/Project" title="">Project</a><ul class="menu clearfix"><li class="first leaf"><a href="/Team:NYU_Gallatin/Project/Cloning" title="">Cloning</a></li>
<li class="leaf"><a href="/Team:NYU_Gallatin/Project/Growing" title="">Growing</a></li>
<li class="leaf"><a href="/Team:NYU_Gallatin/Project/Designing" title="">Designing</a></li>
<li class="leaf"><a href="/Team:NYU_Gallatin/Project/Technology" title="">Technology</a></li>
<li class="last leaf"><a href="/Team:NYU_Gallatin/Project/Socializing" title="">Socializing</a></li>
</ul></li>
</ul>  </div>
</div>
  </div>
      </div></div> <!-- /.section, /#sidebar-first -->
        <div id="igem-content" class="column"><div class="igem-section">
                          <h1 class="title" id="page-title">Cloning:  Transformation Protocol</h1>
                          <div class="tabs"></div>
                          <div class="region region-content">
    <div id="block-system-main" class="block block-system">

    
  <div class="content">
    <div id="node-33" class="node node-page node-full clearfix">

      
  
  <div class="content clearfix">
    <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><ul></ul><ol>Thaw competent cells on ice. Not in hands.</ol><ol>Transfer 50 Âµl of competent cells to 1.5 ml microcentrifuge tube. The competent cells are fragile and sensitive to temperature change, cool the microcentrifuge in the ice for a few seconds first, then be as quick as possible while transferring the cells and return them to the ice.</ol><ol>Add 2 Âµl of the chilled assembly product to competent cells. Mix gently by pipetting up and down or folcking the tube 4-5 times. Do not vortex.</ol><ol>Place the mixture on ice for at least 30 minutes. Do not mix.</ol><ol>Heat shock at 42Â° C for exactly 30 seconds. Do not mix. </ol><ol>Add 950 Âµl of room temperature SOC media to the tube.</ol><ol>Place the tube at 37Â° C for 60 minutes. Shake vigorously (250 rpm) or rotate. </ol><ol>Warm selection plates to 37Â° C. </ol><ol>Spread 100 Âµl of the cells onto the plates.</ol><ol>Incubate overnight at 37Â° C.</ol></div></div></div>  </div>

  
  
</div>
  </div>
</div>
  </div>
          </div></div> <!-- /.section, /#content -->
      </div></div></div> <!-- /#main-inner, /#main, /#main-wrapper -->
   
</nowiki></html>
{{:Team:NYU_Gallatin/Templates/Footer_Content}}
<html>
   
</div></div> <!-- /#page, /#page-wrapper -->
</body></html>
{{:Team:NYU_Gallatin/Templates/Footer}}