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Comparison of the two ordered sequnces. For the forward sequence I had to tel SerialCloner it's Antiparallel: <nowiki> Alignment of Sequence_1: [sequencing_lovtap_reverse.xdna] with Sequence_2: [sequencing_lovtap_forward.xdna] Similarity : 861/1008 (85.42 %) Seq_1 1 ------------------------------------------------------------ 0 Seq_2 1008 AACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAGAGCGCCCAATACGCAAGGAA 949 Seq_1 1 ------------------------------------------------------------ 0 Seq_2 948 ACAGCTATGACCATGTTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAG 889 Seq_1 1 --------------GCCATGG-GAGTTATTAAGAGGTACCGGAATTCCTCAATCGGATTT 45 | | | ||||||||||||||||||||||||||||||||| Seq_2 888 TGAGCGGAAGGCCCATGAGGCCAGTTAATTAAGAGGTACCGGAATTCCTCAATCGGATTT 829 Seq_1 46 GAGCAGGACCTCCTCGAGCCACTGCCGGAGTTCCACAGGGGCGGCTTTCAGGGAGTTGGA 105 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 828 GAGCAGGACCTCCTCGAGCCACTGCCGGAGTTCCACAGGGGCGGCTTTCAGGGAGTTGGA 769 Seq_1 106 TCCCCGTGTAATGGTAGCAATTCCGGCTCCCAGTTCGTTTTTCAGCTCTCTCTGAGACAT 165 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 768 TCCCCGTGTAATGGTAGCAATTCCGGCTCCCAGTTCGTTTTTCAGCTCTCTCTGAGACAT 709 Seq_1 166 TTCGCCTCGGAGCAGCTCCTCCACAATTCGGACTCGTGTGCCCAGTGCTTCCCGTTCATC 225 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 708 TTCGCCTCGGAGCAGCTCCTCCACAATTCGGACTCGTGTGCCCAGTGCTTCCCGTTCATC 649 Seq_1 226 GGGAGTGAGCATGAGATTGAGGAGTGGCAGGTGGAGGTCGTTCTGGTAAGCGTTTTTCAG 285 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 648 GGGAGTGAGCATGAGATTGAGGAGTGGCAGGTGGAGGTCGTTCTGGTAAGCGTTTTTCAG 589 Seq_1 286 GAGGTCCACAAAAGCGGCCTCGTCGATATTCTCGGCGGTTTTTTTGATGAGCATCACGCC 345 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 588 GAGGTCCACAAAAGCGGCCTCGTCGATATTCTCGGCGGTTTTTTTGATGAGCATCACGCC 529 Seq_1 346 CTCTCGTTCAGCAGCATCCCTCACATGTTCAGTGCCATCCAGCTGGACTCCAATAAAGTA 405 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 528 CTCTCGTTCAGCAGCATCCCTCACATGTTCAGTGCCATCCAGCTGGACTCCAATAAAGTA 469 Seq_1 406 CTGGACGTCGCCCTTCTGATCTCTCATAGGCTGGAGGTGGAACAGGTTCCAGAATTTTTT 465 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 468 CTGGACGTCGCCCTTCTGATCTCTCATAGGCTGGAGGTGGAACAGGTTCCAGAATTTTTT 409 Seq_1 466 GCCAGATTTGGTGTAGTTGATGAGCTGGACGGTGACCTCGGTCTGATTATCGATGGCGTC 525 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 408 GCCAGATTTGGTGTAGTTGATGAGCTGGACGGTGACCTCGGTCTGATTATCGATGGCGTC 349 Seq_1 526 CCGGATTTTTCTCACAGTGGCTCTATCGGTTTCAGGTCCCTGGAGGAAGCGACAATTCCG 585 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 348 CCGGATTTTTCTCACAGTGGCTCTATCGGTTTCAGGTCCCTGGAGGAAGCGACAATTCCG 289 Seq_1 586 GCCCAGAATCTCCTCTCTAGAGTACTCAGTGAGCTGGAGGAAGGAATCGGAAGCGAAAAT 645 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 288 GCCCAGAATCTCCTCTCTAGAGTACTCAGTGAGCTGGAGGAAGGAATCGGAAGCGAAAAT 229 Seq_1 646 GATGGGATTATCGGGCAGTCGAGGGTCGGTGATCACAAAGTTTTTCTCAATTCTCTCCAG 705 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 228 GATGGGATTATCGGGCAGTCGAGGGTCGGTGATCACAAAGTTTTTCTCAATTCTCTCCAG 169 Seq_1 706 TGTTGTGGCCAGCATATCCACTTTCCGTTTTTTCTTTGGTCCTCCGTATTCGTCGATCCC 765 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 168 TGTTGTGGCCAGCATATCCACTTTCCGTTTTTTCTTTGGTCCTCCGTATTCGTCGATCCC 109 Seq_1 766 CAGAGCATCGGTGAACATCTGCTCAAACTCGAAGTCGGCCATATCCAGGGCCCCGTAAGG 825 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Seq_2 108 CAGAGCATCGGTGAACATCTGCTCAAACTCGAAGTCGGCCATATCCAGGGCCCCGTAAGG 49 Seq_1 826 GGCAGAATCGTGAGGTGTAAAGCCCATGGTGGCGCCCAAGCTTGGGGAGCTCATGGCGCG 885 |||||||||||||||||||||||||||||||||||||||||| || | Seq_2 48 GGCAGAATCGTGAGGTGTAAAGCCCATGGTGGCGCCCAAGCTGGGAGC------------ 1 Seq_1 886 CCTAGGCCTTGACGGCCTTCCGCCAATTCGCCCTATAGTGAGTCGTATTACGTCGCGCTC 945 Seq_2 0 ------------------------------------------------------------ 1 Seq_1 946 ACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG 1005 Seq_2 0 ------------------------------------------------------------ 1 Seq_1 1006 CCT 1008 Seq_2 0 --- 1 </nowiki>

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