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{{:Team:EPF-Lausanne/Template/Header}} {{:Team:EPF-Lausanne/Template/NotebookHeader}} <!-- Content between here --> == Morning == {{:Team:EPF-Lausanne/Template/LabPresence|Sander}} <!-- {{:Team:EPF-Lausanne/Template/Protocol|None}} --> ; Comments: Took the falcon tubes out of the incubator. There was growth for all concentrations of Spectinomycin (Spc) (slightly less for the higher concentrations, but the difference is not easily noticeable. [[File:Team-EPF-Lausanne_Notebook_29_June_2012_turbidity.jpg]] Our competent cells grew very well on the ampicillin plates with the LovTAP plasmid, but nothing grew on the Spc plates. Successfully prepared the competent cells for the mini-prep in the afternoon (bacteria have been pelleted and put in the freezer). == Afternoon == {{:Team:EPF-Lausanne/Template/Protocol|MiniPrep}} Minipreps were made with bacteria from the plates with growth. {{:Team:EPF-Lausanne/Template/Protocol|Gel}} Around 60 ml of gel prepared. Steps 1 to 7 in protocol. ; Comments: This was done with the LovTAP Read-Out plasmid. The QC hasn't been done yet. <!-- And here --> {{:Team:EPF-Lausanne/Template/Footer}}

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