Team:Austin Texas/Week of July 2 to July 9

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= Notebook for week of July 2 to July 8 =

== Design of Converter Plasmid ==

*Razan
**7/2 - Overlap PCR of Las Promoter and GFP+Terminator for control plasmid.
**7/5 - Amplification PCR of gel extracted band from overlap PCR.

== Design of Reporter Plasmid ==

*Peter
**7/3 - PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.
**7/5 - Made new lab SOC under Aruko's supervision. Transformation of supposedly successful Gibson assembly
**7/6 - Unsuccessful transformation. Made a new second attempt.
**7/7 - Collected 3 successful small colonies from Gibson product assembly, as well as three obviously larger colonies from Gibson control plate which grew. Made cultures for each picked colony.

== Spinach Aptamer ==

== CBB5 Caffeine Inducible Promoters ==

== Inducible Odor ==