Team:WashU/CFlux

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<h1>Carbon Flux</h1>
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In addition to measuring the OD730 of our <i>Synechocystis</i>, we also measured the dry weight in grams of the culture between the third and eighth day. We also measured the dry weight of an equal volume of BG-11 to obtain the dry weight of the <i>Synechocystis</i> alone. Our measurements were made by weighing 10mL of culture in pre-weighed aluminum weigh boats that had been dried for 24 hours in a 90°C oven. In order to calculate the approximate carbon flux from our data, several other plots were created. 
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First, we selected a linear portion of our growth curve, and fitted the curve to a line in order to determine the rate of change of OD730 per day.
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<img src = "http://dl.dropbox.com/u/88390549/carbonflux.png" width=700px>
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Second, a graph of gDW vs OD730 and fitted to a line to determine the rate of change of gDW per OD730.
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<img src = "http://dl.dropbox.com/u/88390549/gdwVSod.png" width = 700px>
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Finally, a graph of the mass of culture vs day was fitted to a line to determine the rate of change of gDW per day. 
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<img src ="http://dl.dropbox.com/u/88390549/culturemassperday.png" width=700px>
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With all these data, we were able to calculate the influx of carbon into the cell. The calculations used to obtain the flux are shown below
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<img src ="http://dl.dropbox.com/u/88390549/eqn1.png">
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Since the molar mass of bicarbonate is 61.017 g/mol, and assuming that bicarbonate accounts for 1% of the total biomass, the influx of bicarbonate into the cell can be calculated to be
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<img src = "http://dl.dropbox.com/u/88390549/Screen%20Shot%202012-10-03%20at%2010.19.23%20PM.png"width=700px>

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