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          	<h6 class="clr-9 p8">NoteBook</h6>
   			<p class="text-3 p3"></p>
            <div class="block-1">
            	<div class="date">27<span>May</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">Week 1.</a></p>
                    Research (both pichia and agrobacterium)- Merhawi, Latara, Khamani, Morgan, Alyssa, Payal, and Mary
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            	<div class="date">03<span>jun</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">Week 2.</a></p>
                    Made YEB medium- Merhawi. Sequencing Plasmids- Alyssa, Payal, and Latara.
Research- Khamani
Finish pgap gene test- Khamani. Had a group meeting!
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            <div class="block-1 top-1 p4">
            	<div class="date">10<span>jun</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 3.</a></p>
                    DNA Transformation - Merhawi and Dafna Made YEB plates- Khamani, Payal, and Mary Made LB agar plates with Kanamycin - Alyssa and Latara Planting - Dafna and Merhawi
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                        <div class="block-1">
            	<div class="date">17<span>jun</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 4.</a></p>
                    Mini prep - Merhawi, Alyssa and Dafna Made SOC medium Merhawi Made some YPD medium- Khamani Preparing cells for transformation (pichia)- Mary Ti Transformation-Innoculated large culture for midi prep, watered plants and electroporation of cells - Latara
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            	<div class="date">24<span>jun</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 5.</a></p>
                    Grew some E. Coli cells- Khamani Culture Ti plasmids, plating and mini plasmid extraction - Alyssa and Merhawi Midi prep - Payal, Khamani, and Mary Gel electrophoresis and gel pictures - Merhawi and Alyssa Restriction digest and made agarose geland PCR- Khamani, Payal, and Mary
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            <div class="block-1 top-1 p4">
            	<div class="date">01<span>jul</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 6.</a></p>
                    Did PCR and did a gel following a restriction digest- Khamani Restriction digest - Payal and Mary PCR, restriction digest and gel - Merhawi and Dafna Found DNA concentration of the midi prep done on pichia- Khamani, Payal, and Mary DNA gel extraction- Khamani Quantification of 20I DNA - Latara Transformation, RFP mini prep, agarose gel, and restriction digest of pORE expression series - Alyssa
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                        <div class="block-1">
            	<div class="date">30<span>jun</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 7.</a></p>
                    Mini prep of 20I- Merhawi Plasmid Extraction/Microscopy transformation of expected vector- 
Chemical transformation- Khamani and Mary made some primers for MCS of pGAP vector - Khamani and Mary Electroporation- Khamani and Payal
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            	<div class="date">15<span>jul</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 8.</a></p>
                    Payal, Mary, Khamani- Miniprep, restriction digest, gel electrophoresis of pGAP. Merhawi and Alyssa- Miniprep plus quantitative analysis, and culture colonies from plates
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            <div class="block-1 top-1 p4">
            	<div class="date">22<span>jul</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 9.</a></p>
                    Alyssa, Merhawi-Library research, wiki, plasmid digest. Khamani and Brandi- Vectors Payal & Mary- Plate to broth transfer of transformed pGap and Miniprep. Alyssa - midi prep of pORE vector
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                        <div class="block-1">
            	<div class="date">29<span>jul</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 10.</a></p>
                    Payal, Mahesh, Mary,- Electrocompetent cells of E. coli, ran gels of midi and mini prep and Chlorophenicol research Latara- Research and planting
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            <div class="block-1 top-1">
            	<div class="date">05<span>aug</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 11.</a></p>
                    Latara- Planting and Research Merhawi- Research
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            <div class="block-1 top-1 p4">
            	<div class="date">12<span>aug</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 12.</a></p>
                    Khamani- Primer Stock (resuspended dry oligos) Research for another binary vector - Dafna
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                        <div class="block-1">
            	<div class="date">19<span>aug</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 13.</a></p>
                    Merhawi- Watered plants Khamani- PCR of pGAP and restriction digest. Mary, Payal, Khamani, Krystle- Gel Electrophoresis
                </div>
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            <div class="block-1 top-1">
            	<div class="date">26<span>aug</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 14.</a></p>
                    Latara- Vector Design Khamani Robinson- Gel Extraction MCS and gel analysis Krystle- Ligation of linker
                </div>
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            </div>
            <div class="block-1 top-1 p4">
            	<div class="date">02<span>sept</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 15.</a></p>
                    Payal, Mary- LB broth, picked transformed cultures put into broth. Alyssa- Mini Prep Dafna - cell cultures of E4 vector
Latara- Miniprep of 20I DNA and quantification and restriction digest Krystle- Miniprep of pGap with linker
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            </div>
                        <div class="block-1">
            	<div class="date">09<span>sept</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 16.</a></p>
                    Merhawi, Kelsey, Alyssa Dafna - transformation of cell cultures from E4 vector and primer design for pPZP500 vector Latara- Miniprep and Restriction Digest of E4 vector Khamani- Club fair, wiki, cultures, research, miniprep Morgan- Miniprep with Khamani Alyssa - restriction digest and mini prep
                </div>
                <div class="clear"></div>
            </div>
            <div class="block-1 top-1">
            	<div class="date">16<span>sept</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 17.</a></p>
                    Khamani and Krystle- Restriction digest and pGap digest with linker gel Payal, Mary, Amish- wiki posters,broth Alyssa-Mini prep of LB broth + Ecoli cells with Austria Vector cultures, Restiction digest with EcoRI enzyme of mini prep samples and gel Electrophoresis. Gel showed no bands.- PCR of ecoli with Austria vector colonies (picked from LB/Kan plates), Gel electrophoresis of finished PCR samples, prepared LB/Spectinomycin agar Dafna:Poured LB/Spectinomycin plates Latara - transformation with Sarah and quanitfication
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            <div class="block-1 top-1 p4">
            	<div class="date">25<span>sept</span></div>
                <div class="extra-wrap">
                	<p><a href="#" class="link-1">week 18.</a></p>
                    Payal and Mary- PCR of mini preps and ran gel Dr. Brewer- Ran gel of PCR Krystle- PCR of mini preps and Midiprep of pGap alpha z with linker Khamani- YPD with zeocin medium plates and broth, ligation of RFP into pGap. Transformation into pichia Dafna - made LB agar plates with Spectinomycin and plate transformed pPZP500 vector Merhawi - transformation of pPZP500 vector Latara - PCR to get Kanamycin resistance out
                </div>
                <div class="clear"></div>
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