Team:University College London/LabBook/Week3

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==Monday (18.6.12)==

Aim – Day 1 of Generating Competent Cells: In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.

In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.

'''Method'''

<html><div class="protocol protocol-Competency">Day 1 of Generating Competent Cells</div><div class="protocolContent"></html>{{:Team:University_College_London/Protocols/Competency}}<html></div></html>


== Tuesday (19.6.12) ==

'''Aim – Picking Colonies:''' Today we check the colony formation from yesterday’s culture, and pick a colony for inoculation

'''Method'''

<html><div class="protocol protocol-Competency">Day 2 of Generating Competent Cells</div><div class="protocolContent"></html>{{:Team:University_College_London/Protocols/Competency2}}<html></div></html>


== Wednesday (20.6.12) ==


'''Aim – Permeabilisation:''' Incubating Cells in the presences of CaCl<sub>2</sub> will prepare the cell wall, such that it becomes permeable to DNA. 

'''Method'''

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<html><div class="protocol protocol-Generic">Bla bla bla</div><div class="protocolContent"></html>{{:Team:University_College_London/Protocols/Competency2}}<html></div></html>


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