Team:TU Darmstadt/Protocols/GC-MS/GC-Protocol

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==INSTRUMENT CONTROL PARAMETERS==
===Control Information===


*Sample Inlet      :  GC
*Injection Source  :  GC ALS
*Mass Spectrometer :  Enabled

====Oven====
*Equilibration Time                 0.25 min
*Oven Program                         On
    55 °C for 0.5 min
    then 30 °C/min to 250 °C for 3 min
*Run Time                             10 min

===Front Injector===
*Syringe Size                         10 µL
*Injection Volume                      1 µL
*Injection Repetitions                 1
*Solvent A Washes (PreInj)             2
*Solvent A Washes (PostInj)            2
*Solvent A Volume                      5 µL
*Solvent B Washes (PreInj)             2
*Solvent B Washes (PostInj)            2
*Solvent B Volume                      5 µL
*Sample Washes                         1
*Sample Wash Volume                    1 µL
*Sample Pumps                          2
*Dwell Time (PreInj)                   0 min
*Dwell Time (PostInj)                  0 min
*Solvent Wash Draw Speed             300 µL/min
*Solvent Wash Dispense Speed        6000 µL/min
*Sample Wash Draw Speed              300 µL/min
*Sample Wash Dispense Speed         6000 µL/min
*Injection Dispense Speed           6000 µL/min
*Viscosity Delay                       1 sec
*Sample Depth Disabled
===Back Injector===
*Front SS Inlet He
*Mode                             Pulsed Splitless
*Heater                               On    270 °C
*Pressure                             On    0.54761 bar
*Total Flow                           On    54 mL/min
*Septum Purge Flow                    On    3 mL/min
*Gas Saver                            On    15 mL/min After 3 min
*Injection Pulse Pressure              1 bar Until 0.5 min
*Purge Flow to Split Vent             50 mL/min at 2 min


===Thermal Aux 1 {MSD Transfer Line}===
*Heater                               On
*Temperature Program                  On
320 °C for 0 min
*Run Time                             10 min

===Column #1===
*HP-5MS  5% Phenyl Methyl Silox: 0001
*HP-5MS  5% Phenyl Methyl Silox
*325 °C: 30 m x 250 µm x 0.25 µm
*In: Front SS Inlet He
*Out: Vacuum

*(Initial)                            55 °C
*Pressure                         0.54761 bar
*Flow                                  1 mL/min
*Average Velocity                 36.534 cm/sec
*Holdup Time                      1.3686 min
*Flow Program                        Off
*    1 mL/min for 0 min
*Run Time                             10 min