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{{:Team:EPF-Lausanne/Template/Header}} {{:Team:EPF-Lausanne/Template/NotebookHeader}} <!-- Content between here --> == pSB1C3-LovTAP and pCEP4-HA-RO colony PCR == {{:Team:EPF-Lausanne/Template/Protocol|PCR}} A colony PCR was performed on the (hopefully) correctly ligated LovTAP BioBrick from the day before, and on the pCEP4-HA-RO plates that were stored in the fridge. == Gel electrophoresis == {{:Team:EPF-Lausanne/Template/Protocol|Gel}} ; pSB1C3-LovTAP gel: [[File:Team-EPF-Lausanne LovTAPbb.JPG|600px]] This is a working BioBrick, all the ligations have worked! ; pCEP4-HA-RO gel: [[File:Team-EPF-Lausanne pCEP4-HA-RO.JPG|600px]] The colony PCR has failed. It turned out that the primers we had designed for this check were wrong. We ordered new ones and recultivated the colonies during the wait. == Maxipreps == {{:Team:EPF-Lausanne/Template/Protocol|MaxiPrep}} Maxipreps were made from overnight cultures of pHY42, pcDNA3.1(+)-LovTAP, and pGL4.30-GFP. == Western Blot result from 4.sep.12 == {{:Team:EPF-Lausanne/Template/Protocol|Western_Blot}} VP16 with CHO cell lysate, to check how it looks in cells. [[File:Team-EPF-Lausanne-4.sep.12 result.png|600px]] Looks like the size of the VP16 protein we are testing our WB is around 35kDa. This does not correspond to the 25kDa that the manual promises, we have no idea why. == Overnight cultures == {{:Team:EPF-Lausanne/Template/Protocol|PreparePlasmidExtraction}} Overnight cultures of the pSB1C3-RFP plasmid for miniprepping. It was cultured on chloramphenicol. == Soldering == The assembly of the reactor plates was completed without major issues. <!-- And here --> {{:Team:EPF-Lausanne/Template/Footer}}

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