Team:EPF-Lausanne/Protocol/PCR

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1X Mastermix 20μl reaction

# Water 20μl - x
# HF-Buffer (5x) 4μl
# DMSO (optional) 0.6μl
#dNTPs 0.4μl
#Primers (50μM) 
## Forward 0.2μl
## Reverse 0.2 μl
#Template (10ng/μl) 0.5μl
#Phusion HF polymerase 0.2μl


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