File:PCR Purified eGFP & plsr.JPG

From 2012.igem.org

6/21/12 EtBr Gel 2% Agarose Lane 1: NEB 2-log ladder (1 ug loaded) Lane 2: PCR of eGFP using plsr_XhoI Fwd & eGFP_EcoRI Rev primers w/ 55C annealing temp. Lane 3: PCR of plsr using plsr_BglII Fwd & plsr_XhoI Rev primers w/ 55C annealing temp. Lane 4: PCR of eGFP using plsr_XhoI Fwd & eGFP_EcoRI Rev primers w/ 50C annealing temp. Lane 5: PCR of plsr using plsr_BglII Fwd & plsr_XhoI Rev primers w/ 50C annealing temp. Lane6/7: Null Lane 8: 1kb ladder left at rt o/n

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current17:38, 21 June 2012Thumbnail for version as of 17:38, 21 June 2012579×709 (81 KB)Qiaop (Talk | contribs) (6/21/12 EtBr Gel 2% Agarose Lane 1: NEB 2-log ladder (1 ug loaded) Lane 2: PCR of eGFP using plsr_XhoI Fwd & eGFP_EcoRI Rev primers w/ 55C annealing temp. Lane 3: PCR of plsr using plsr_BglII Fwd & plsr_XhoI Rev primers w/ 55C annealing temp. Lane 4: PCR )

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Camera manufacturer Canon
Camera model Canon PowerShot S90
Exposure time 1/20 sec (0.05)
F Number f/2
ISO speed rating 800
Date and time of data generation 13:58, 21 June 2012
Lens focal length 6 mm
Orientation Normal
Software used Microsoft Windows Photo Viewer 6.1.7600.16385
File change date and time 13:25, 21 June 2012
Y and C positioning 2
Exif version 2.21
Date and time of digitizing 13:58, 21 June 2012
Image compression mode 3
Shutter speed 4.3125
Aperture 2
Exposure bias 0
Maximum land aperture 2
Metering mode Pattern
Flash Flash did not fire, compulsory flash suppression
DateTimeOriginal subseconds 00
DateTimeDigitized subseconds 00
Color space sRGB
Focal plane X resolution 12,493.150684932
Focal plane Y resolution 12,493.150684932
Focal plane resolution unit inches
Sensing method One-chip color area sensor
Custom image processing Normal process
Exposure mode Auto exposure
White balance Auto white balance
Digital zoom ratio 1
Scene capture type Standard
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